Payner T D, Drake R L, Saker D M, Shipley M T
Department of Anatomy and Cell Biology, University of Cincinnati College of Medicine, OH 45267-0521.
Brain Res Bull. 1987 Aug;19(2):287-90. doi: 10.1016/0361-9230(87)90095-5.
Sucrose density centrifugation has been used to characterize the relative levels of AChE molecular forms in different parts of the brain, during development, or in various disease states. We have examined the influence of various tissue or sample storage and handling techniques on the abundance of the 4S and 10S molecular forms of AChE in rat forebrain. Our results demonstrate that freezing either a subcellular fraction or the intact tissue causes dramatic shifts in the level of the 4S and 10S molecular forms as compared to the values obtained in fresh tissue. Total AChE activity was unchanged suggesting that 4S and 10S forms are equally active and that 4S AChE is easily dissociated from 10S. These observations suggest that 4S and 10S molecular forms in brain are extremely labile and that great care should be taken when studying the factors that regulate these forms.
蔗糖密度离心法已被用于表征大脑不同部位、发育过程中或各种疾病状态下乙酰胆碱酯酶（AChE）分子形式的相对水平。我们研究了各种组织或样品储存及处理技术对大鼠前脑AChE 4S和10S分子形式丰度的影响。我们的结果表明，与新鲜组织中获得的值相比，冷冻亚细胞组分或完整组织会导致4S和10S分子形式的水平发生显著变化。总AChE活性未变，这表明4S和10S形式具有同等活性，且4S AChE很容易从10S中解离出来。这些观察结果表明，大脑中的4S和10S分子形式极其不稳定，在研究调节这些形式的因素时应格外小心。
