简化的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳用于未浓缩尿液，具有更高的分辨率和检测灵敏度。
Simplified sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unconcentrated urine with enhanced resolution and detection sensitivity.
作者信息
Marshall T, Williams K M
机构信息
Biology Department, University of Ulster, Coleraine, Northern Ireland.
出版信息
Clin Chem. 1987 Oct;33(10):1886-7.
We applied a simple sodium dodecyl sulfate-polyacrylamide gel electrophoresis method to urine. The method, developed for serum protein analysis (Clin Chem 1984;30:475-9), has a high sample throughput and gives excellent resolution with unconcentrated urine. It clearly distinguishes and characterizes proteinuric urine (7.5 microL) by Coomassie Blue staining and gives complex silver-stained patterns with nonproteinuric urine (2 microL). The former is recommended for routine clinical screening, the latter for research purposes.
我们将一种简单的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳方法应用于尿液检测。该方法是为血清蛋白分析开发的（《临床化学》1984年；30:475 - 479），具有高通量样本处理能力，对未浓缩尿液也能给出出色的分辨率。通过考马斯亮蓝染色，它能清晰地区分并鉴定蛋白尿尿液（7.5微升），对非蛋白尿尿液（2微升）则给出复杂的银染图谱。前者推荐用于常规临床筛查，后者用于研究目的。
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