Kim Han-Jun, Kim Na Na, Han Jeonghoon, Park Heung-Sik, Kang Do-Hyung, Choi Young-Ung
Marine Bio-Resources Research Unit, Korea Institute of Ocean Science & Technology (KIOST), Busan, Republic of Korea.
National Institute of Fisheries Science, Changwon, Republic of Korea.
J Exp Zool A Ecol Integr Physiol. 2023 Apr;339(3):302-309. doi: 10.1002/jez.2679. Epub 2023 Jan 17.
This study analyzed the relationship between the lunar phase and the reproductive cycle of Pinctada margaritifera inhabiting Weno Island, Chuuk Lagoon, Micronesia. We measured indicators of maturity (gonadosomatic index [GSI] and sexual maturation-related genes) and investigated changes in the gonadal maturity stages (GMS) of P. margaritifera over lunar cycle. GSI was higher around the full moon. GMS of P. margaritifera were classified as the early gametogenesis stage, ripe and spawning stage, and spent and degenerating stage. A large percentage of oysters was observed in the ripe and spawning stage at the first quarter moon in female and the full moon in male as well as in the spent and degenerating stages at the third quarter moon in both sexes. In addition, the expression of doublesex- and mab-3-related transcription factor 2 (DMRT2) in the male P. margaritifera black-lip pearl oyster was the highest during the full and third quarter moon phases, whereas no difference in expression was observed with the lunar phase in females. In contrast, the expression of vitellogenin (VTG) was the highest in female P. margaritifera during the first and third quarters. No difference in expression was observed according to the lunar phase in males. The results suggest that the lunar phase directly affects the expression of sexually mature gonads in P. margaritifera black-lip pearl oyster.
本研究分析了密克罗尼西亚楚克泻湖韦诺岛的珠母贝生殖周期与月相之间的关系。我们测量了成熟度指标（性腺指数[GSI]和与性成熟相关的基因），并研究了珠母贝在月周期内性腺成熟阶段（GMS）的变化。满月前后GSI较高。珠母贝的GMS分为配子发生早期阶段、成熟和产卵阶段以及耗尽和退化阶段。在雌性的上弦月和雄性的满月时，观察到很大比例的牡蛎处于成熟和产卵阶段，而在两性的下弦月时处于耗尽和退化阶段。此外，雄性珠母贝黑唇珍珠牡蛎中双性和mab-3相关转录因子2（DMRT2）的表达在满月和下弦月阶段最高，而在雌性中未观察到其表达随月相的差异。相反，卵黄蛋白原（VTG）的表达在雌性珠母贝的上弦月和下弦月期间最高。在雄性中未观察到其表达随月相的差异。结果表明，月相对珠母贝黑唇珍珠牡蛎性成熟性腺的表达有直接影响。
