Iwakawa M, Tofilon P J, Hunter N, Stephens L C, Milas L
Department of Experimental Radiotherapy, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Clin Exp Metastasis. 1987 Oct-Dec;5(4):289-300. doi: 10.1007/BF00120724.
N-Methylformamide (NMF), a cell-differentiating agent, was assessed for its antitumor activity against a fibrosarcoma (FSA), a hepatocarcinoma (HCA-I) and a mammary carcinoma (MCA-K), syngeneic to C3Hf/Kam mice. Tumors were grown as solitary tumors in the leg or as artificial or spontaneous micrometastases in the lung. NMF, at a dose of 300 mg/kg, was administered i.p. daily for 6 to 18 days. NMF slowed the growth of FSA and HCA-I tumors and totally inhibited the growth of the MCA-K tumor. However, the effect was transient; tumors resumed their pretreatment growth rate upon cessation of the treatment. Histologically, MCA-K tumors treated with NMF (300 mg/kg daily for six days) underwent considerable cell depopulation and reduction in mitotic activity. The number of artificial metastases, as well as the incidence and the number of spontaneous metastases, were markedly reduced by NMF. This resulted in a prolongation of the survival of mice that had artificial metastases of MCA-K tumor. The in vitro clonogenicity of MCA-K, but not of FSA or HCA-I cells, was reduced. However, in vivo reduction of MCA-K cell clonogenicity was minimal, if any. Thus, NMF is effective in restricting the growth of both solitary tumors and metastases, but the degree of response is highly dependent on tumor type.
N-甲基甲酰胺（NMF）是一种细胞分化剂，对C3Hf/Kam小鼠同基因的纤维肉瘤（FSA）、肝癌（HCA-I）和乳腺癌（MCA-K）进行了抗肿瘤活性评估。肿瘤以腿部孤立肿瘤或肺部人工或自发微转移的形式生长。以300mg/kg的剂量腹腔注射NMF，每天给药，持续6至18天。NMF减缓了FSA和HCA-I肿瘤的生长，并完全抑制了MCA-K肿瘤的生长。然而，这种效果是短暂的；治疗停止后，肿瘤恢复到治疗前的生长速度。组织学上，用NMF治疗的MCA-K肿瘤（每天300mg/kg，持续6天）经历了显著的细胞减少和有丝分裂活性降低。NMF显著减少了人工转移灶的数量以及自发转移灶的发生率和数量。这导致了患有MCA-K肿瘤人工转移灶的小鼠存活期延长。MCA-K细胞的体外克隆形成能力降低，但FSA或HCA-I细胞未出现这种情况。然而，在体内，MCA-K细胞克隆形成能力的降低即使有也是最小的。因此，NMF在限制孤立肿瘤和转移灶的生长方面是有效的，但反应程度高度依赖于肿瘤类型。
