Gupta Ajay, Adarsh Tarun, Manchanda Vikas, Sasmal Pijus K, Gupta Shalini
School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Department of Chemical Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
Dalton Trans. 2023 Jan 31;52(5):1188-1192. doi: 10.1039/d2dt03554e.
The highly contagious COVID-19, caused by the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed using reverse transcription polymerase chain reaction (RT-PCR). However, despite being highly sensitive, RT-PCR is also time consuming and quite complex, which limits its use for point-of-care (POC) testing. We have developed a simple single-step fluorescence assay for SARS-CoV-2 RNA detection based on the principle of aggregation-induced emission (AIE) using iridium complexes. Our smartly designed iridium probes fluorescently "turn-on" in the presence of SARS-CoV-2 RNA and give specific results at room temperature within 10 min. The lower limit of detection (LOD) is 1.84 genome copies per reaction, and the sensitivity and specificity of the assay in 20 clinical samples are found to be 90% and 80%, respectively.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）爆发引起的极具传染性的2019冠状病毒病（COVID-19），通常使用逆转录聚合酶链反应（RT-PCR）进行诊断。然而，尽管RT-PCR高度灵敏，但它也耗时且相当复杂，这限制了其在即时检测（POC）中的应用。我们基于铱配合物的聚集诱导发光（AIE）原理，开发了一种用于SARS-CoV-2 RNA检测的简单单步荧光测定法。我们精心设计的铱探针在存在SARS-CoV-2 RNA时荧光“开启”，并在室温下10分钟内给出特定结果。检测下限（LOD）为每个反应1.84个基因组拷贝，并且该测定法在20个临床样本中的灵敏度和特异性分别为90%和80%。
