Department of Zoology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Division of Science and Technology, Department of Zoology, University of Education, Lahore, Pakistan.
Biopreserv Biobank. 2024 Feb;22(1):21-28. doi: 10.1089/bio.2022.0029. Epub 2023 Jan 18.
Bacterial contamination may occur in feces during collection and processing of semen. Bacteria not only compete for nutrients with spermatozoa but also produce toxic metabolites and endotoxins and affect sperm quality. The aim of the present study was to investigate the effect of antibiotic supplementation on the sperm quality of Indian red jungle fowl, estimation and isolation of bacterial species and their antibiotic sensitivity. Semen was collected and initially evaluated, diluted, and divided into six experimental extenders containing gentamicin (2.5 μg/mL), kanamycin (31.2 μg/mL), neomycin (62.5 mg/mL), penicillin (200 U/mL), and streptomycin (250 μg/mL), and a control having no antibiotics were cryopreserved and semen quality was evaluated at post-dilution, post-cooling, post-equilibration, and post-thawing stages (Experiment 1). A total aerobic bacterial count was carried out after culturing bacteria (Experiment 2) and subcultured for antibiotic sensitivity (Experiment 3). It was shown that penicillin-containing extender improved semen quality (sperm motility, plasma membrane integrity, viability, and acrosomal integrity) compared with the control and other extenders having antibiotics. The bacteria isolated from semen were , spp., and spp. Antibiotic sensitivity results revealed that shows high sensitivity toward neomycin, kanamycin, and penicillin. spp. shows high sensitivity toward streptomycin, neomycin, and penicillin. spp. shows high sensitivity toward kanamycin and penicillin. It was concluded that antibiotics added to semen extender did not cause any toxicity and maintained semen quality as that of untreated control samples, and penicillin was identified as most effective antibiotic. It is recommended that penicillin can be added to the semen extender for control of bacterial contamination without affecting the semen quality of Indian red jungle fowl.
在采集和处理精液过程中，粪便中可能会发生细菌污染。细菌不仅与精子竞争营养物质，还会产生有毒代谢物和内毒素，从而影响精子质量。本研究旨在探讨抗生素添加对印度红原鸡精液质量的影响，估计和分离细菌种类及其抗生素敏感性。采集精液并进行初步评估，稀释后分为六组实验，分别为含有庆大霉素（2.5μg/ml）、卡那霉素（31.2μg/ml）、新霉素（62.5mg/ml）、青霉素（200U/ml）和链霉素（250μg/ml）的扩展剂，对照组不添加抗生素进行冷冻保存，并在稀释后、冷却后、平衡后和解冻后评估精液质量（实验 1）。实验 2 培养细菌后进行总需氧菌计数，实验 3 进行细菌亚培养和抗生素敏感性检测。结果表明，与对照组和其他含抗生素的扩展剂相比，含青霉素的扩展剂可提高精液质量（精子活力、质膜完整性、活力和顶体完整性）。从精液中分离出的细菌为 、 spp. 和 spp. 抗生素敏感性结果显示， 对新霉素、卡那霉素和青霉素高度敏感。 spp. 对链霉素、新霉素和青霉素高度敏感。 spp. 对卡那霉素和青霉素高度敏感。结论是，添加到精液扩展剂中的抗生素不会造成任何毒性，并保持精液质量与未处理的对照样品相同，青霉素被鉴定为最有效的抗生素。建议在精液扩展剂中添加青霉素，以控制细菌污染，而不影响印度红原鸡的精液质量。
