Department of Zoology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, Rawalpindi, Pakistan.
Division of Science and Technology, Department of Zoology, University of Education, Lahore, Pakistan.
Reprod Domest Anim. 2023 Sep;58(9):1199-1206. doi: 10.1111/rda.14419. Epub 2023 Jul 7.
The Indian red jungle fowl population is decreasing in its natural habitat. Its conservation through semen cryopreservation with sufficient live sperm recovery rate is requisite where ascorbic acid could play significant role to mitigate cryo-incited injuries. The objective was to elucidate the effect of ascorbic acid on freezability of Indian red jungle fowl sperm. Pooled semen was aliquoted and diluted (1:5) with red fowl extender having ascorbic acid: 0.0 (control), 1.0, 2.0 and 4.0 mM. Diluted samples were cryopreserved and semen quality was assessed at post-dilution, cooling, equilibration and freeze-thawing stages. Sperm metabolic status, antioxidant potential and lipid peroxidation were studied at post-dilution and freeze-thawing. Sperm motility did not differ (p > .05) in experimental extenders and control at post-dilution and cooling; however, it was recorded higher (p < .05) with ascorbic acid at 2.0 mM compared with other levels at post-equilibration and post-thawing stage. Sperm viability, plasma membrane and acrosome intactness were recorded higher (p < .05) with 2.0 mM ascorbic acid compared with other concentrations of ascorbic acid at all stages of cryopreservation. Sperm metabolic status and antioxidant potential were recorded higher (p < .05), while lipid peroxidation was recorded lowest (p < .05) with 2.0 mM ascorbic acid compared with 1.0, 4.0 mM and control. In conclusion, ascorbic acid at 2.0 mM in red fowl extender improves quality, metabolic status and antioxidant potential of frozen Indian red jungle fowl semen through ameliorating lipid peroxidation.
印度红原鸡的自然栖息地数量正在减少。为了保护它，需要通过精液冷冻保存来提高精子回收率，而抗坏血酸可以在减轻冷冻损伤方面发挥重要作用。本研究的目的是阐明抗坏血酸对印度红原鸡精子冷冻能力的影响。将混合精液等分并与含有抗坏血酸的红原鸡稀释液（0.0mM（对照）、1.0mM、2.0mM 和 4.0mM）以 1:5 的比例稀释。稀释后的样本进行冷冻保存，并在稀释后、冷却、平衡和冻融阶段评估精液质量。在稀释后和冻融后研究精子代谢状态、抗氧化能力和脂质过氧化。在稀释后和冷却阶段，实验稀释液和对照中精子的运动能力没有差异（p>0.05）；然而，在平衡后和冻融后阶段，2.0mM 抗坏血酸组的精子运动能力记录较高（p<0.05），与其他水平相比。在所有冷冻保存阶段，2.0mM 抗坏血酸组的精子存活率、质膜和顶体完整性记录较高（p<0.05），与其他抗坏血酸浓度相比。与 1.0mM、4.0mM 和对照相比，2.0mM 抗坏血酸组的精子代谢状态和抗氧化能力记录较高（p<0.05），而脂质过氧化记录较低（p<0.05）。综上所述，红原鸡稀释液中 2.0mM 的抗坏血酸通过改善脂质过氧化，提高了冷冻印度红原鸡精子的质量、代谢状态和抗氧化能力。
