Chen Yi-Juan, Zhao Jie, Jiang Jie-Xian, Wan Nian-Feng
Eco-environmental Protection Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Engineering Research Centre of Low-carbon Agriculture, 201403, Shanghai, China.
Shanghai Pudong New District Agro-Technology Extension Center, 66 Changxin East Road, 201201, Shanghai, China.
Exp Appl Acarol. 2023 Jan;89(1):61-84. doi: 10.1007/s10493-022-00772-1. Epub 2023 Jan 19.
Natural acaricides are potential biorational mite control alternatives to conventional chemical acaricides. However, little is known about the molecular mechanism of defense response to natural acaricides in mites. We previously reported significant acaricidal properties of ethyl oleate (EO) against Tetranychus cinnabarinus (here referred to as a sibling species of two-spotted spider mite, Tetranychus urticae), a highly polyphagous pest devastating crops in fields and greenhouses worldwide. In this study, we explored the molecular responses of T. cinnabarinus exposed to EO using RNA-Seq and differentially expressed gene (DEG) analysis. A total of 131, 185, and 154 DEGs were identified in T. cinnabarinus after 1, 6, and 24 h of EO treatment. In addition, 36 putative detoxification-related DEGs, including 10 cytochrome P450s (P450s), three glutathione S-transferases (GSTs), nine UDP-glycosyltransferases (UGTs), eight esterases (ESTs), and six ATP-binding cassette transporters (ABC transporters), were identified. Interestingly, the upregulation of these detoxification-related genes might be the main defense response of T. cinnabarinus exposed to EO. A quantitative real-time PCR analysis indicated that the expression profiles of 19 random DEGs were consistent with the RNA-Seq results. These findings serve as valuable information for a better understanding of the acaricide-mite interaction and molecular mechanisms involved in the defense response of T. cinnabarinus against EO.
天然杀螨剂是传统化学杀螨剂潜在的生态合理的杀螨替代物。然而，关于螨类对天然杀螨剂防御反应的分子机制知之甚少。我们之前报道了油酸乙酯（EO）对朱砂叶螨（这里指二斑叶螨的近缘种）具有显著的杀螨特性，朱砂叶螨是一种多食性害虫，在全球田间和温室中破坏农作物。在本研究中，我们使用RNA测序和差异表达基因（DEG）分析，探索了暴露于EO的朱砂叶螨的分子反应。在EO处理1、6和24小时后，在朱砂叶螨中分别鉴定出131、185和154个差异表达基因。此外，还鉴定出36个推定的与解毒相关的差异表达基因，包括10个细胞色素P450（P450s）、3个谷胱甘肽S-转移酶（GSTs）、9个UDP-糖基转移酶（UGTs）、8个酯酶（ESTs）和6个ATP结合盒转运蛋白（ABC转运蛋白）。有趣的是，这些与解毒相关基因的上调可能是朱砂叶螨暴露于EO后的主要防御反应。定量实时PCR分析表明，19个随机选择的差异表达基因的表达谱与RNA测序结果一致。这些发现为更好地理解杀螨剂与螨类的相互作用以及朱砂叶螨对EO防御反应所涉及的分子机制提供了有价值的信息。
