包涵体的抗菌应用

Antimicrobial Applications of Inclusion Bodies.

机构信息

Translational Vectorology Research Unit, Children's Medical Research Institute, The University of Sydney, Westmead, NSW, Australia.

Department of Ruminant Production, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, Spain.

出版信息

Methods Mol Biol. 2023;2617:257-269. doi: 10.1007/978-1-0716-2930-7_19.

DOI:10.1007/978-1-0716-2930-7_19

PMID:36656531

Abstract

A broad number of inclusion bodies (IBs) potential uses, including biocatalysis, biocompatible nanomaterials, and nanopills for biomedicine, have been described so far. Recently, it has also been shown that they can also be used as antimicrobial agents. Here, we describe the protocol used to produce and purify IBs with antimicrobial activity at desirable yields and also an optimized and simple methodology to determine the antimicrobial activity of IBs against bacterial strains.

摘要

迄今为止，已经描述了大量包涵体（IB）的潜在用途，包括生物催化、生物相容性纳米材料和用于生物医药的纳米丸。最近，还表明它们也可用作抗菌剂。在这里，我们描述了一种生产和纯化具有所需产量的抗菌活性的包涵体的方案，以及一种优化且简单的方法来测定包涵体对细菌菌株的抗菌活性。