J Vis Exp. 2023 Jan 19(191). doi: 10.3791/6525.
An erratum was issued for: Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis. The Protocol and Representative Results sections were updated. Step 3.2.1 of the Protocol has been updated from: On day "4", prepare 0.4% hapten: TNCB in an acetone-ethanol mixture (ratio 1:1). Prepare the solution just before use in a glass vial and protect it from light by covering the vial with aluminum foil. to: On day "4", prepare 0.4% hapten: TNCB in an acetone: olive oil mixture (ratio 1:1). Prepare the solution just before use in a glass vial and protect it from light by covering the vial with aluminum foil. Figure 1 of the Representative Results has been updated from: Figure 1: Induction of CHS. Sensitization, challenge, and ear measurement.
CHS = contact hypersensitivity reaction; TNCB = 2,4,6-trinitrochlorobenzene. Please click here to view a larger version of this figure. to: Figure 1: Induction of CHS. Sensitization, challenge, and ear measurement.
CHS = contact hypersensitivity reaction; TNCB = 2,4,6-trinitrochlorobenzene. Please click here to view a larger version of this figure. Figure 2 of the Representative Results has been updated from: Figure 2: Adoptive transfer of the CHS-effector cells.
ALNs =axillary and inguinal lymph nodes; CHS = contact hypersensitivity reaction; i.v. = intravenously; SPLs = spleens; TNCB = 2,4,6-trinitrochlorobenzene. Please click here to view a larger version of this figure. to: Figure 2: Adoptive transfer of the CHS-effector cells.
ALNs =axillary and inguinal lymph nodes; CHS = contact hypersensitivity reaction; i.v. = intravenously; SPLs = spleens; TNCB = 2,4,6-trinitrochlorobenzene. Please click here to view a larger version of this figure.
