Department of Applied Chemistry, Delhi Technological University, Delhi 42, India.
Department of Applied Physics, Delhi Technological University, Delhi 42, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Apr 15;291:122345. doi: 10.1016/j.saa.2023.122345. Epub 2023 Jan 11.
In this article, highly fluorescent phosphorus(V) corrole was synthesised which was then combined with CdSe quantum dots (QDs) in order to study Förster resonance energy transfer (FRET) mechanism between CdSe QDs (donor) and phosphorus corrole (acceptor). Spectral overlap between QD's emission profile and corrole's absorption profile was found to be significant enough to result into Förster resonance energy transfer (FRET). The UV-vis spectrum experienced increase in the absorption bands on addition of phosphorus corrole to CdSe QDs suggesting QD-corrole conjugation. In the steady state fluorescence measurements, emission spectrum observed quenching in the fluorescence intensity of prepared CdSe QDs on addition of phosphorus corrole. Likewise, in case of time-resolved fluorescence measurements it was noticed that the CdSe QD's lifetime was greatly quenched by the presence of a corrole acceptor. Stern-Volmer plot was made to show quenching in this case was dynamic in nature. Based on the results of UV-vis, steady state and time-resolved fluorescence measurements the plausible mechanism behind such observations is considered to be FRET.
在本文中，合成了具有高荧光的磷(V)叶啉，然后将其与 CdSe 量子点 (QDs) 结合，以研究 CdSe QDs（供体）和磷叶啉（受体）之间的Förster 共振能量转移 (FRET) 机制。发现 QD 的发射谱与叶啉的吸收谱之间的光谱重叠足够显著，导致 Förster 共振能量转移 (FRET)。在加入磷叶啉后，UV-vis 光谱的吸收带增加，表明 QD-叶啉的共轭。在稳态荧光测量中，观察到在加入磷叶啉后，制备的 CdSe QDs 的荧光强度发生猝灭。同样，在时间分辨荧光测量中，注意到 CdSe QD 的寿命由于存在叶啉受体而大大猝灭。制作 Stern-Volmer 图以表明这种情况下的猝灭是动态的。基于 UV-vis、稳态和时间分辨荧光测量的结果，认为这种观察背后的合理机制是 FRET。
