Tianjin Key Laboratory of Composite & Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China; Ningbo Yinzhou Chinaust Automobile Fittings Corp. Ltd., Ningbo 315142, China.
Tianjin Key Laboratory of Composite & Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China; School of Mechanical and Electrical Engineering, Shaoxing University, Shaoxing 312000, China; Ningbo Yinzhou Chinaust Automobile Fittings Corp. Ltd., Ningbo 315142, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Apr 15;291:122338. doi: 10.1016/j.saa.2023.122338. Epub 2023 Jan 13.
Autophagy is the controlled breakdown of cellular components that dysfunctional or nonessential, and the decomposition products are further recycled and synthesized for the normal physiological activities of cells. Lysosomal autophagy has been implicated in cancer, neurological disorders, Parkinson's disease, etc. Therefore, it is necessary to develop a fluorescent probe that can clearly describe the process of lysosomal autophagy. However, there are currently limited fluorescent probes for ratiometric monitoring of the autophagic process in dual channels. To solve this problem, a fluorescent probe based on spiropyran with lysosomal targeting and pH response for ratiometric monitoring the autophagy process of lysosomes were designed. The sensitive response of the probe to pH in vitro was verified by UV and fluorescence spectrum tests. Meanwhile, the probe demonstrated the ability to monitor the intracellular pH fluctuations. In addition, the application of Lyso-SD in the field of anti-counterfeiting has been proposed based on the obvious photoluminescence ability of Lyso-SD under UV irradiation.
自噬是细胞内功能失调或非必需成分的受控分解，其分解产物进一步回收和合成，以维持细胞的正常生理活动。溶酶体自噬与癌症、神经紊乱、帕金森病等有关。因此，有必要开发一种荧光探针，可以清楚地描述溶酶体自噬过程。然而，目前用于双通道比率监测自噬过程的荧光探针有限。为了解决这个问题，设计了一种基于螺吡喃的荧光探针，具有溶酶体靶向性和对 pH 的响应，用于比率监测溶酶体的自噬过程。通过紫外和荧光光谱试验验证了探针对 pH 的敏感响应。同时，该探针还具有监测细胞内 pH 波动的能力。此外，还提出了基于 Lyso-SD 在紫外光照射下具有明显的磷光能力，在防伪领域的应用。
