环丙氟菌胺导致 SdhB 和 SdhC 点突变与耐药性的关系。
Resistance Risk and Resistance-Related Point Mutation in SdhB and SdhC of Cyclobutrifluram in .
机构信息
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, 3 Taicheng Road, Yangling712100, ShaanxiChina.
Syngenta (China) Investment Co., Ltd., 567 Bocheng Road, Shanghai200120, China.
出版信息
J Agric Food Chem. 2023 Feb 1;71(4):1886-1895. doi: 10.1021/acs.jafc.2c08022. Epub 2023 Jan 19.
Cyclobutrifluram is a novel succinate dehydrogenase inhibitor (SDHI) developed by Syngenta and helps to inhibit . Here, the potential for cyclobutrifluram resistance in and the resistance mechanism involved were evaluated. Baseline sensitivity of to cyclobutrifluram was determined with a mean EC value of 0.0248 μg/mL. Fungicide adaption generated five resistant mutants, which possess a comparable or a slightly impaired fitness compared to corresponding parental isolates. This indicates that the resistance risk of to cyclobutrifluram might be moderate. Cyclobutrifluram-resistant isolates also demonstrated resistance to pydiflumetofen but sensitivity to carbendazim, phenamacril, tebuconazole, fludioxonil, or pyraclostrobin. Additionally, point mutations H248Y in FpSdhB and A83V or R86K in FpSdhC were found in cyclobutrifluram-resistant mutants. Molecular docking and overexpression transformation assay revealed that FpSdhB and FpSdhC or FpSdhC confer the resistance of to cyclobutrifluram.
环丙氟灵是先正达公司开发的一种新型琥珀酸脱氢酶抑制剂（SDHI），有助于抑制 。在这里，评估了 和涉及的抗性机制对环丙氟灵的抗性潜力。用平均 EC 值为 0.0248μg/mL 确定了 对环丙氟灵的基础敏感性。杀菌剂适应产生了五个抗性突变体，与相应的亲本分离株相比，它们具有相当或略低的适应性。这表明 对环丙氟灵的抗性风险可能是中等的。环丙氟灵抗性分离株也表现出对吡氟酰草胺的抗性，但对多菌灵、苯菌灵、戊唑醇、氟啶胺或吡唑醚菌酯敏感。此外，在环丙氟灵抗性 突变体中发现了 FpSdhB 中的 H248Y 和 FpSdhC 中的 A83V 或 R86K 点突变。分子对接和过表达转化试验表明，FpSdhB 和 FpSdhC 或 FpSdhC 赋予了 对环丙氟灵的抗性。
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