Green engineering of TMC-CMS nanoparticles decorated graphene sheets for targeting M. tuberculosis.

Our current work intends to primarily engineer a new type of antibacterial composite by preparing a highly biocompatible graphene sheet decorated with TMC-CMS IPNs nanoparticles utilizing one-pot, green, cost-effective ultrasonication approach. The microstructure of as-formed materials was chemically confirmed using various analytical techniques such as H-NMR, FTIR, UV/vis, SEM, and TEM. TEM data has proved the formation of uniformly distributed TCNPs on graphene surfaces with a small particle size of 22 nm compared with that of pure nanoparticles (30 nm). The inhibitory activity of these developed materials was examined against the growth of three different M. tuberculosis pathogens and in a comparison with the isoniazid drug as a standard anti-tuberculosis drug. The TCNPs@GRP composite attained MIC values of 0.98, 3.9, and 7.81 μg/mL for inhibiting the growth of sensitive, MDR, and XDR M. tuberculosis pathogens compared to the bare TCNPs (7.81, 31.25, >125 μg/mL) and the isoniazid drug (0.24, 0, 0 μg/mL), respectively. This reveals a considerable synergism in the antituberculosis activity between TCNPs and graphene nanosheets. Cytotoxicity of the TCNPs@GRP was examined against normal lung cell lines (WI38) and was found to have cell viability of 100% with the concentration range of 0.98-7.81 μg/mL.