Kim Han-Sem, Jang JunHwee, Oh Jun-Sung, Lee Eun-Jung, Han Cheol-Min, Shin Ueon Sang
Department of Nano-biomedical Science & BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 31116, Republic of Korea.
Department of Nano-biomedical Science & BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 31116, Republic of Korea.
Carbohydr Polym. 2023 Mar 1;303:120473. doi: 10.1016/j.carbpol.2022.120473. Epub 2022 Dec 17.
A combination of hydrogel materials, and therapeutic agents have been actively reported to facilitate bone defect healing. However, conventionally hydrogels using cross-linker would result in low stability of the hydrogel itself, loss of agents during cross-linking, and complexity of use. In this study, alendronate was tethered to an AlA to improve its bone healing and drug-loading stability. AlA was further functionalized with Ca (AlACa). A mixture of AlACa and alginate formed AlAA hydrogel. The gelation time of AlAA was sufficient for injecting into the defect site. The hydrogel stiffness was controlled, while the stress-relaxation time was fixed. In vitro cell tests demonstrated that the AlAA promoted proliferation and differentiation behaviors. In particular, AlAA showed the best mechanical stiffness with appropriate stress-relaxation and cellular behavior, indicating that it would be beneficial as a scaffold in the bone tissue engineering field.
水凝胶材料与治疗剂的组合已被积极报道可促进骨缺损愈合。然而，传统上使用交联剂的水凝胶会导致水凝胶本身稳定性低、交联过程中试剂损失以及使用复杂。在本研究中，阿仑膦酸盐与AlA相连以改善其骨愈合和药物负载稳定性。AlA进一步用Ca进行功能化（AlACa）。AlACa和藻酸盐的混合物形成AlAA水凝胶。AlAA的凝胶化时间足以注入缺损部位。水凝胶的硬度得到控制，而应力松弛时间固定。体外细胞测试表明，AlAA促进了增殖和分化行为。特别是，AlAA表现出最佳的机械硬度，具有适当的应力松弛和细胞行为，表明它作为骨组织工程领域的支架将是有益的。
