Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Argentina.
Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC-CONICET), and Fundación para Investigaciones Biológicas & Aplicadas (FIBA), Mar del Plata, Argentina.
J Food Sci. 2023 Mar;88(3):1075-1088. doi: 10.1111/1750-3841.16464. Epub 2023 Jan 19.
The aim of this work is to produce bioactive films suitable for aerobic packaging applications by combining the bioactivity of Spirulina platensis protein concentrate (PC; 1% and 2% w/w), the sustainable nature of bovine gelatin (Ge), and sodium alginate dialdehyde (ADA, 5% w/w) as Schiff base crosslinking agent. PC was obtained by an optimized acid-base extraction process and characterized. PC showed a dose-dependent radical scavenging activity (RSA; IC = 24.3 mg/L) related to its high content of C-phycocyanin and total phenolic compounds (32.44 ± 1.37 mg gallic acid equivalents per gram of PC). As a general trend, crosslinking decreased the water solubility, improved mechanical properties, and helped improve RSA of Ge-ADA-PC films. Ge-5ADA-2PC film recorded best compromise between solubility (only 33.6%), high UV barrier (0.134% transmittance at 400 nm), reasonable extensibility (217.00 ± 2.34%), tensile strength (3.50 ± 0.43 MPa), water vapor permeability (2.00 ± 0.17 × 10 kg·m/m ·Pa·s), and RSA (44.70 ± 2.19%). Wrapping hake fillets in this filmdelayed lipid oxidation during storage under refrigerated conditions for 11 days, maintaining the thiobarbituric acid index below 0.5 mg malonaldehyde/kg muscle. Results suggest that Ge-ADA-PC films have potential as aerobic packaging materials for oxidation-sensitive food. PRACTICAL APPLICATION: The combination of gelatin, alginate dialdehyde and Spirulina platensis protein concentrate gave rise to fully biobased films with reduced water solubility and enhanced antioxidant activity, which were able to delay the secondary lipid oxidation of refrigerated seafood. This study also shows the potential of cyanobacteria as renewable resources of high-value ingredients for the design of active and intelligent aerobic packaging solutions.
本工作旨在通过结合螺旋藻蛋白浓缩物（PC；1%和 2%w/w）的生物活性、牛明胶（Ge）的可持续性和海藻酸钠二醛（ADA，5%w/w）作为席夫碱交联剂，制备适用于需氧包装应用的生物活性薄膜。通过优化的酸碱提取工艺获得 PC 并对其进行了表征。PC 表现出剂量依赖性自由基清除活性（RSA；IC = 24.3 mg/L），这与其高含量的 C-藻蓝蛋白和总酚类化合物（每克 PC 中含有 32.44 ± 1.37 毫克没食子酸当量）有关。一般来说，交联降低了水溶性，改善了机械性能，并有助于提高 Ge-ADA-PC 薄膜的 RSA。Ge-5ADA-2PC 薄膜在水溶性（仅 33.6%）、高紫外线阻隔（400nm 时透光率为 0.134%）、合理的延展性（217.00 ± 2.34%）、拉伸强度（3.50 ± 0.43 MPa）、水蒸气透过率（2.00 ± 0.17×10 -3 kg·m/m·Pa·s）和 RSA（44.70 ± 2.19%）之间取得了最佳折衷。在冷藏条件下储存 11 天期间，用这种薄膜包装牙鳕鱼片可以延缓脂肪氧化，使硫代巴比妥酸指数保持在肌肉 0.5 毫克以下。结果表明，Ge-ADA-PC 薄膜具有作为氧化敏感食品的需氧包装材料的潜力。实际应用：明胶、海藻酸钠二醛和螺旋藻蛋白浓缩物的结合产生了水溶性降低且抗氧化活性增强的全生物基薄膜，能够延缓冷藏海鲜的二次脂质氧化。本研究还表明了蓝藻作为高价值成分的可再生资源在设计活性和智能需氧包装解决方案方面的潜力。
