Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, 77030, USA.
The Third Xiangya Hospital, Central South University, Changsha, 410008, P. R. China.
Adv Sci (Weinh). 2023 Mar;10(8):e2204544. doi: 10.1002/advs.202204544. Epub 2023 Jan 19.
Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC-dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics-based single-cell migration platform is described that enables high-throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.
树突状细胞 (DC) 迁移是其执行适应性免疫功能的一个基本步骤。研究 DC 迁移特性对于开发依赖 DC 的过敏治疗、疫苗和癌症免疫疗法至关重要。在这里，描述了一种基于微流控的单细胞迁移平台，该平台可实现高通量和精确的双向细胞迁移分析。它还允许选择性回收具有不同迁移潜力的细胞亚群。使用这个微流控平台，研究了 DC 对不同趋化因子和抑制剂的迁移反应，定量描述了 DC 的迁移模式，并回收了具有不同迁移潜力的 DC 亚群，用于差异基因表达分析。该平台为精确表征细胞迁移和潜在治疗调节剂的发现开辟了道路。
