Hyrien O, Debatisse M, Buttin G, de Saint Vincent B R
Unité de Génétique Somatique (UA CNRS 361), Institut Pasteur, Paris, France.
EMBO J. 1987 Aug;6(8):2401-8. doi: 10.1002/j.1460-2075.1987.tb02518.x.
We have identified, in the amplified domain of adenylate deaminase (AMPD) overproducing Chinese hamster fibroblasts, a 2.6 kb recombinogenic DNA region which is frequently involved in amplification-associated rearrangements. The nucleotide sequence reveals a mosaic organization of four Alu-equivalent repeats of the B1 and B2 families and eight long A + T-rich DNA segments. Part of this region is enriched with long imperfect palindromes. The center of one palindrome contains a putative topoisomerase I cleavage site and this site defines the position of a novel junction which was formed by illegitimate recombination with anther A + T-rich DNA sequence located far apart on the amplified DNA. These findings and their significance are discussed in the context of related data from other systems and in the light of current models for eukaryotic DNA recombination, replication and organization.
我们在过量产生腺苷酸脱氨酶（AMPD）的中国仓鼠成纤维细胞的扩增区域中，鉴定出一个2.6 kb的重组DNA区域，该区域经常参与与扩增相关的重排。核苷酸序列显示出B1和B2家族的四个Alu等效重复序列和八个长的富含A+T的DNA片段的镶嵌组织。该区域的一部分富含长的不完全回文序列。一个回文序列的中心包含一个推定的拓扑异构酶I切割位点，该位点定义了一个新连接点的位置，该连接点是通过与扩增DNA上相距很远的另一个富含A+T的DNA序列进行非法重组而形成的。这些发现及其意义将结合来自其他系统的相关数据，并根据当前关于真核DNA重组、复制和组织的模型进行讨论。
