由RNA聚合酶III体外转录中国仓鼠DNA中Alu型分散重复序列产生的低分子量RNA在体内也能找到。
Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo.
作者信息
Haynes S R, Jelinek W R
出版信息
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6130-4. doi: 10.1073/pnas.78.10.6130.
An Alu-type dispersed repeat previously identified in a cloned fragment of Chinese hamster DNA [Haynes, S. R., Toomey, T. P., Leinwand, L. & Jelinek, W. R. (1981) Mol. Cell. Biol. 1, 573-583] serves as a template for cell-free transcription of discrete low molecular weight RNAs by RNA polymerase III [RNA nucleotidyltransferase (DNA-directed), EC 2.7.7.6]. A class of analogous RNAs has been isolated from growing Chinese hamster cells by hybridization of total low molecular weight nuclear RNAs to the cloned DNA fragment from which cell-free transcription occurs. Two-dimensional analysis of RNase digestion products of these RNAs suggests that they are transcribed from multiple members of the Alu-type dispersed repeat family.
先前在中国仓鼠DNA的一个克隆片段中鉴定出的一种Alu型分散重复序列[海恩斯，S.R.，图米，T.P.，莱因万德，L.和杰利内克，W.R.（1981年）《分子细胞生物学》1，573 - 583]作为RNA聚合酶III[RNA核苷酸转移酶（DNA指导），EC 2.7.7.6]进行无细胞转录离散低分子量RNA的模板。通过将总低分子量核RNA与发生无细胞转录的克隆DNA片段杂交，从生长中的中国仓鼠细胞中分离出了一类类似的RNA。对这些RNA的核糖核酸酶消化产物进行二维分析表明，它们是从Alu型分散重复序列家族的多个成员转录而来的。
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