Department of Clinical Sciences Malmö, Lund University, Clinical Research Center, Malmö, Sweden.
Department of Cardiology, Malmö, Skåne University Hospital, Lund University, Malmö, Sweden.
Methods Mol Biol. 2023;2619:91-98. doi: 10.1007/978-1-0716-2946-8_7.
Bomb pulse refers to the sharp increase of the atmospheric levels of the carbon isotope C due to nuclear testing during the late 1950s and early 1960s. After the nuclear test ban, atmospheric C levels decreased exponentially because of the diffusion and equilibration with the ocean and biosphere, as well as the incorporation indirectly into all living cells. Thus, assessing the anthropogenic C serves as an isotopic chronometer providing the opportunity to estimate the age of a tissue and its components with a relatively high precision comparing it with the atmospheric levels at a certain time stamp.This protocol describes the use of the bomb pulse dating as a retrospective tracer of tissue components such as the glycosaminoglycans (GAGs) and subsequent proteoglycans turnover using accelerator mass spectrometry.
炸弹脉冲是指由于 20 世纪 50 年代末和 60 年代初的核试验，大气中的碳同位素 C 水平急剧上升。核试验禁令之后，由于与海洋和生物圈的扩散和平衡以及间接掺入所有活细胞，大气中的 C 水平呈指数级下降。因此，评估人为 C 可作为同位素计时器，提供了一种机会，可以通过与特定时间标记的大气水平进行比较，以相对较高的精度来估计组织及其成分的年龄。本方案描述了使用炸弹脉冲定年来作为组织成分（如糖胺聚糖（GAG）和随后的蛋白聚糖周转）的回溯示踪剂的方法，该方法使用加速器质谱法。
