Ivanov P L, Akulichev A V, Ryskov A P
Genetika. 1987 Aug;23(8):1349-59.
Recombinant cDNA clones containing the repetitive sequences of major retroposon families--ID, B2 and L1--were isolated from the cDNA bank of rat brain. Their organization was studied by sequencing and hybridization analysis. It was shown for L1 that: 1) its transcripts are mainly found in the fraction of poly(A) +mRNA; 2) there is a population of full-length elements present in the rat genome. We also tried to estimate the copy number of these elements. Some aspects of their organization are discussed.
从大鼠脑cDNA文库中分离出了包含主要反转座子家族(ID、B2和L1)重复序列的重组cDNA克隆。通过测序和杂交分析研究了它们的结构。对于L1来说,结果表明:1)其转录本主要存在于多聚腺苷酸(poly(A))+mRNA组分中;2)大鼠基因组中存在全长元件群体。我们还试图估算这些元件的拷贝数。讨论了它们结构的一些方面。