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[大鼠骨髓细胞中基因组重复序列的表达:L1元件转录本的cDNA拷贝的克隆与特性]

[Expression of genome repetitive sequences in rat bone marrow cells: cloning and characteristics of cDNA-copies of L1 element transcripts].

作者信息

Ivanov P L, Akulichev A V, Ryskov A P

出版信息

Genetika. 1987 Aug;23(8):1349-59.

PMID:3666447
Abstract

Recombinant cDNA clones containing the repetitive sequences of major retroposon families--ID, B2 and L1--were isolated from the cDNA bank of rat brain. Their organization was studied by sequencing and hybridization analysis. It was shown for L1 that: 1) its transcripts are mainly found in the fraction of poly(A) +mRNA; 2) there is a population of full-length elements present in the rat genome. We also tried to estimate the copy number of these elements. Some aspects of their organization are discussed.

摘要

从大鼠脑cDNA文库中分离出了包含主要反转座子家族(ID、B2和L1)重复序列的重组cDNA克隆。通过测序和杂交分析研究了它们的结构。对于L1来说,结果表明:1)其转录本主要存在于多聚腺苷酸(poly(A))+mRNA组分中;2)大鼠基因组中存在全长元件群体。我们还试图估算这些元件的拷贝数。讨论了它们结构的一些方面。

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