在还原型谷胱甘肽和过氧化氢存在的情况下，由辣根过氧化物酶或血红素催化生成激发态物种。

Generation of excited species catalyzed by horseradish peroxidase or hemin in the presence of reduced glutathione and H2O2.

作者信息

Medeiros M H, Wefers H, Sies H

机构信息

Institut für Physiologische Chemie I, Universität Düsseldorf, Federal Republic of Germany.

出版信息

Free Radic Biol Med. 1987;3(2):107-10. doi: 10.1016/s0891-5849(87)80004-7.

DOI:10.1016/s0891-5849(87)80004-7

PMID:3666514

Abstract

Horseradish peroxidase (HRP) (EC 1.11.1.7) catalyzes the oxidation of reduced glutathione. This reaction is accompanied by light emission, which is attributed to the generation of singlet oxygen. The chemiluminescence is directly related to thiyl radical formation, as deduced from the correlation between the time course of HRP-compound II formation and light emission in the presence of different amounts of H2O2. Superoxide dismutase has an inhibitory effect on the chemiluminescence without affecting the HRP-compound II formation. This indicates the direct involvement of superoxide radicals in the production of photoemissive species. Replacement of HRP by hemin is also accompanied by chemiluminescence.

摘要

辣根过氧化物酶（HRP）（EC 1.11.1.7）催化还原型谷胱甘肽的氧化。该反应伴随着发光现象，这归因于单线态氧的产生。从在不同量过氧化氢存在下HRP-化合物II形成的时间进程与发光之间的相关性推断，化学发光与硫自由基的形成直接相关。超氧化物歧化酶对化学发光有抑制作用，而不影响HRP-化合物II的形成。这表明超氧自由基直接参与了光发射物种的产生。用血红素替代HRP也会伴随着化学发光。