Casajús Victoria, Howe Kevin, Fish Tara, Civello Pedro, Thannhauser Theodore, Li Li, Gómez Lobato María, Martínez Gustavo
Instituto de Fisiología Vegetal (INFIVE) UNLP-CONICET, 113 and 61, 1900, La Plata, Argentina.
Robert W. Holley Center for Agriculture and Health, USDA-Agricultural Research Service, Cornell University, Ithaca, NY, 14853, USA.
Plant Physiol Biochem. 2023 Feb;195:322-329. doi: 10.1016/j.plaphy.2023.01.012. Epub 2023 Jan 12.
Broccoli is a vegetable appreciated by consumers for its nutritional properties, particularly for its high glucosinolate (GLS) content. However, broccoli shows a high rate of senescence during postharvest and the GLS content in inflorescences decreases sharply. Usually, postharvest studies on broccoli focus on inflorescences, ignoring the other tissues harvested such as the stems and main stalk. In this work, GLS metabolism in whole heads of broccoli (including inflorescences, small stems and stalk) was analysed during postharvest senescence. The content of GLS content, expression of GLS metabolic genes, and expression of GLS transport-associated genes were measured in the three parts of harvested broccoli. A marked decrease in the content of all GLSs was detected in inflorescences, but an increase in the stems and stalk. Also, decreased expressions of GLS biosynthesis and degradation genes were detected in all tissues analysed. On the other hand, an increase in the expression of one of the genes involved in GLS transport was observed. These results suggest that GLSs would be transported from inflorescences to stems during postharvest senescence. From a commercial point of view, broccoli stems are usually discarded and not used as food. However, the accumulation of GLSs in the stems is an important factor to consider when contemplating potential commercial use of this part of the plant.
西兰花是一种因其营养特性而受到消费者喜爱的蔬菜，尤其是其高含量的硫代葡萄糖苷（GLS）。然而，西兰花在采后表现出较高的衰老速率，花球中的GLS含量急剧下降。通常，西兰花的采后研究集中在花球上，而忽略了收获的其他组织，如茎和主茎。在这项研究中，分析了西兰花整株（包括花球、小茎和主茎）在采后衰老过程中的GLS代谢。测定了收获的西兰花三个部分中GLS的含量、GLS代谢基因的表达以及GLS转运相关基因的表达。在花球中检测到所有GLS的含量显著下降，但在茎和主茎中增加。此外，在所有分析的组织中均检测到GLS生物合成和降解基因的表达降低。另一方面，观察到参与GLS转运的一个基因的表达增加。这些结果表明，在采后衰老过程中，GLS会从花球转运到茎中。从商业角度来看，西兰花的茎通常被丢弃，不作为食物使用。然而，当考虑该植物这一部分的潜在商业用途时，茎中GLS的积累是一个需要考虑的重要因素。
