Mendez-Sotelo Braulio Josue, López-Jácome Luis Esaú, Colín-Castro Claudia A, Hernández-Durán Melissa, Martínez-Zavaleta Maria Guadalupe, Rivera-Buendía Frida, Velázquez-Acosta Consuelo, Rodríguez-Zulueta Ana Patricia, Morfín-Otero Maria Del Rayo, Franco-Cendejas Rafael
División de Infectología, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Mexico City 14389, Mexico.
Oficina de Apoyo Sistemático para la Investigación Superior, Subdirección de Investigación Clínica, Instituto Nacional de Cardiología, Mexico City 14080, Mexico.
Antibiotics (Basel). 2023 Jan 6;12(1):96. doi: 10.3390/antibiotics12010096.
The identification of carbapenemase-producing Enterobacterales and is important for treating and controlling hospital infections. The recommended methods for their identification require a long waiting time, technical training, and expertise. Lateral flow immunoassays such as NG-Test CARBA 5 overcome these needs. We analyzed 84 clinical isolates of carbapenem-resistant Enterobacterales and from four different hospitals in a two-year period. Antimicrobial resistance patterns were confirmed with the broth dilution method. Evaluation of KPC, VIM, NDM, IMP, and OXA-48-like enzymes was performed and compared to NG-Test CARBA 5 and phenotypic assays. Enterobacterales represented 69% of isolates and represented 31%. Carbapenemase-producing strains were 51 (88%) of Enterobacterales and 23 (88.4%) of ; 20 (34%) and 23 (88%) were Class B ß-lactamases, respectively. The NG-Test CARBA 5 assay for Enterobacterales showed high sensitivity (98%), specificity (100%), and PPV (100%); however, it did not for . The Kappa concordance coefficient was 0.92 for Enterobacterales and 0.52 for . NG-Test CARBA 5 is a fast and easy-to-use assay. In Enterobacterales, we found excellent agreement in our comparison with molecular tests. Despite the low agreement in , we suggest that this test could be used as a complementary tool.
鉴定产碳青霉烯酶的肠杆菌科细菌对于治疗和控制医院感染很重要。推荐的鉴定方法需要较长的等待时间、技术培训和专业知识。诸如NG-Test CARBA 5之类的侧向流动免疫测定法克服了这些需求。我们在两年时间里分析了来自四家不同医院的84株耐碳青霉烯肠杆菌科细菌临床分离株。用肉汤稀释法确认了抗菌药物耐药模式。对KPC、VIM、NDM、IMP和OXA-48样酶进行了评估，并与NG-Test CARBA 5和表型测定法进行了比较。肠杆菌科细菌占分离株的69%，[此处原文缺失相关菌名]占31%。产碳青霉烯酶菌株在肠杆菌科细菌中占51株（88%），在[此处原文缺失相关菌名]中占23株（88.4%）；分别有20株（34%）和23株（88%）为B类β-内酰胺酶。针对肠杆菌科细菌的NG-Test CARBA 5检测显示出高灵敏度（98%）、特异性（100%）和阳性预测值（100%）；然而，对[此处原文缺失相关菌名]则不然。肠杆菌科细菌的Kappa一致性系数为0.92，[此处原文缺失相关菌名]的为0.52。NG-Test CARBA 5是一种快速且易于使用的检测方法。在肠杆菌科细菌中，我们发现与分子检测相比一致性极佳。尽管在[此处原文缺失相关菌名]方面一致性较低，但我们建议该检测可作为一种补充工具。
