Tsuboi S, Pederson J E, Toris C B
Department of Ophthalmology, University of Minnesota, Minneapolis, Minnesota 55455.
Invest Ophthalmol Vis Sci. 1987 Nov;28(11):1788-94.
The integrity of the RPE barrier function in retinal detachment was studied in vitro. The retinal pigment epithelium (RPE)-choroid tissue was isolated from cynomolgus monkey eyes with acute (less than 1 hr), subacute (1-2 weeks), and chronic (8-20 months) retinal detachments, and clamped between Ussing-type chambers. Electrical characteristics and choroid-to-retina permeability to carboxyfluorescein were determined. In the HEPES-buffered bathing solution, transepithelial potential difference and resistance in eyes with acute retinal detachments (0.2 mV and 134 ohm-cm2, respectively) were significantly lower than subacute (7.9 and 350) and chronic (10.4 and 348) retinal detachments. Furthermore, the permeability was increased five-fold in acute retinal detachments with respect to subacute and chronic retinal detachments, indicating a breakdown of the RPE barrier in acute retinal detachment. No statistical difference was found between subacute and chronic retinal detachments. In this animal model, RPE barrier function is destroyed at the onset of retinal detachment, but recovers in a week or two, and is maintained in the chronic stage. Histological examination revealed that RPE recovery was accomplished by RPE proliferation and hyperplasia.
在体外研究了视网膜脱离中视网膜色素上皮（RPE）屏障功能的完整性。从患有急性（少于1小时）、亚急性（1 - 2周）和慢性（8 - 20个月）视网膜脱离的食蟹猴眼中分离出视网膜色素上皮（RPE）-脉络膜组织，并夹在Ussing型小室之间。测定了电特性以及脉络膜对羧基荧光素的视网膜通透性。在HEPES缓冲的浴液中，急性视网膜脱离眼的跨上皮电位差和电阻（分别为0.2 mV和134 ohm-cm²）显著低于亚急性（7.9和350）和慢性（10.4和348）视网膜脱离眼。此外，急性视网膜脱离时的通透性相对于亚急性和慢性视网膜脱离增加了五倍，表明急性视网膜脱离时RPE屏障被破坏。亚急性和慢性视网膜脱离之间未发现统计学差异。在这个动物模型中，RPE屏障功能在视网膜脱离开始时被破坏，但在一两周内恢复，并在慢性期维持。组织学检查显示，RPE的恢复是通过RPE的增殖和增生完成的。
