Garnier M, Iskra M L, Bove J M
Laboratoire de Biologie, Cellulaire et Moléculaire, Institut Nationale de la Recherche Agronomique, Pont de la Maye, France.
Isr J Med Sci. 1987 Jun;23(6):691-5.
A 6-week-old female BALB/c mouse was immunized with a preparation of partially purified sieve tubes of periwinkle (Catharantus roseus) plants infected with the apple proliferation mycoplasma-like organism. After fusion of the immunized spleen cells with myeloma cells, the supernatant fluids of the resulting hybridoma cultures were screened for the presence of antibodies in a differential avidin-biotin enzyme-linked immunosorbent assay (ELISA) using sieve tube preparations from either healthy or infected periwinkles as antigens. One hybridoma clone, giving a negative ELISA with the sieve tube preparation from healthy plants and a positive ELISA with that infected ones, was selected. This hybridoma also gave a negative ELISA when tested against sieve tube preparations from periwinkles affected by citrus greening or Spiroplasma citri, but a positive ELISA when tested against phyllody-affected sieve-tube preparations.
用感染苹果增殖类支原体生物的长春花（Catharantus roseus）植物的部分纯化筛管制剂对一只6周龄的雌性BALB/c小鼠进行免疫。将免疫的脾细胞与骨髓瘤细胞融合后，使用来自健康或感染长春花的筛管制剂作为抗原，通过差异抗生物素蛋白-生物素酶联免疫吸附测定（ELISA）筛选所得杂交瘤培养物的上清液中是否存在抗体。选择了一个杂交瘤克隆，该克隆对来自健康植物的筛管制剂进行ELISA检测呈阴性，而对感染植物的筛管制剂进行ELISA检测呈阳性。当用受柑橘黄龙病或柑橘螺原体影响的长春花筛管制剂进行检测时，该杂交瘤也呈ELISA阴性，但当用受叶变病影响的筛管制剂进行检测时呈ELISA阳性。
