Bryzgunova Olga, Bondar Anna, Ruzankin Pavel, Tarasenko Anton, Zaripov Marat, Kabilov Marsel, Laktionov Pavel
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Sobolev Institute of Mathematics, Siberian Branch Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Cancers (Basel). 2023 Jan 9;15(2):431. doi: 10.3390/cancers15020431.
The locus-specific methylation of three genes (GSTP1, RNF219, and KIAA1539, also known as FAM214B) in the total pool of blood cell-free DNA, including cell-free DNA from plasma and cell-surface-bound DNA, of patients with prostate cancer and healthy donors was studied on the MiSeq platform. Our study found a higher methylation index of loci for total cell-free DNA compared with cell-free DNA. For total cell-free DNA, the methylation of GSTP1 in each of the 11 positions provided a complete separation of cancer patients from healthy donors, whereas for cell-free DNA, there were no positions in the three genes allowing for such separation. Among the prostate cancer patients, the minimum proportion of GSTP1 genes methylated in any of the 17 positions was 12.1% of the total circulated DNA fragments, and the minimum proportion of GSTP1 genes methylated in any of the 11 diagnostically specific positions was 8.4%. Total cell-free DNA was shown to be more convenient and informative as a source of methylated DNA molecules circulating in the blood than cell-free DNA.
在MiSeq平台上，研究了前列腺癌患者和健康供体血细胞游离DNA总库（包括来自血浆的游离DNA和细胞表面结合的DNA）中三个基因（GSTP1、RNF219和KIAA1539，也称为FAM214B）的位点特异性甲基化。我们的研究发现，与游离DNA相比，总游离DNA位点的甲基化指数更高。对于总游离DNA，GSTP1在11个位置中的每个位置的甲基化都能将癌症患者与健康供体完全区分开，而对于游离DNA，三个基因中没有任何位置能实现这种区分。在前列腺癌患者中，GSTP1基因在17个位置中任何一个位置甲基化的最小比例为循环DNA片段总数中的12.1%，在11个诊断特异性位置中任何一个位置甲基化的最小比例为8.4%。与游离DNA相比，总游离DNA作为血液中循环的甲基化DNA分子来源更方便且信息更丰富。
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