Lnc-AS Regulates -Mediated Adipocyte Differentiation and Lipogenesis in Pigs through miR-503-5p.

Animal fat deposition has a significant impact on meat flavor and texture. However, the molecular mechanisms of fat deposition are not well understood. Lnc-AS is a naturally occurring transcript that is abundant in porcine adipose tissue. Here, we focus on the regulatory role of lnc-AS in promoting preadipocyte proliferation and adipocyte differentiation. By overexpressing or repressing lnc expression, we found that lnc-AS promoted the transcription of its host gene , a regulator of adipocyte differentiation. In addition, we predicted the region of lnc-AS that binds to miR-503-5p and showed by dual luciferase assay that lnc-AS acts as a sponge to absorb miR-503-5p. Interestingly, miR-503-5p also targets and represses expression and helps regulate porcine preadipocyte proliferation and differentiation. Taken together, these results show that lnc-AS upregulates expression by absorbing miR-503-5p, suggesting a potential regulatory mechanism based on competing endogenous RNAs. Finally, we explored lnc-AS and expression in adipose tissue and found that both molecules were expressed at significantly higher levels in fatty pig breeds compared to lean pig breeds. In summary, we identified the mechanism by which lnc-AS regulates porcine preadipocyte proliferation and differentiation, contributing to our understanding of the molecular mechanisms of lipid deposition in pigs.