Ichioka Hiroaki, Saito Urara, Shintani-Ishida Kaori, Shirahase Takahira, Idota Nozomi, Kanamura Narisato, Ikegaya Hiroshi
Department of Forensic Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465, Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.
Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465, Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.
Diagnostics (Basel). 2023 Jan 14;13(2):311. doi: 10.3390/diagnostics13020311.
In a regular autopsy, blood and organs are used to quantify drug and toxicant concentrations; however, specimens such as blood cannot be collected from highly decomposed corpses, making the quantification of drug and toxicants impossible. This study aimed to estimate the blood carbamazepine (CBZ) concentration from teeth, a part of the human body that is best preserved after death. We sampled teeth and blood of rats administered CBZ. The correlation between the tooth and serum CBZ concentrations was analyzed. Rats were euthanized after CBZ administration and kept at 22 °C for 0 to 15 days before sampling the teeth and measuring the CBZ concentration. Undecalcified, fresh, frozen sections of rat teeth were prepared, and CBZ localization was evaluated. CBZ concentrations in both teeth and cardiac blood peaked at 60 min after administration and increased in a dose-dependent manner. CBZ concentration in teeth did not substantially change after death, with high CBZ distribution being observed in the pulp cavity. The tooth and serum CBZ concentrations were highly correlated, suggesting that the measurement of toxicant concentration in sampled teeth would allow for the estimation of blood toxicant concentration in highly decomposed corpses.
在常规尸检中，血液和器官被用于量化药物和毒物浓度；然而，对于高度腐败的尸体无法采集血液等样本，因此无法对药物和毒物进行量化。本研究旨在通过牙齿（人体死后保存最完好的部位之一）来估算血液中卡马西平（CBZ）的浓度。我们采集了给予CBZ的大鼠的牙齿和血液样本。分析了牙齿和血清中CBZ浓度之间的相关性。给予CBZ后对大鼠实施安乐死，并在22℃下保存0至15天，然后采集牙齿样本并测量CBZ浓度。制备大鼠牙齿的未脱钙新鲜冰冻切片，并评估CBZ的定位情况。给药后60分钟时，牙齿和心脏血液中的CBZ浓度均达到峰值，且呈剂量依赖性增加。死后牙齿中的CBZ浓度基本未发生变化，在牙髓腔中观察到较高的CBZ分布。牙齿和血清中的CBZ浓度高度相关，这表明通过测量采集到的牙齿中的毒物浓度可以估算高度腐败尸体血液中的毒物浓度。
