Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 28003 Madrid, Spain.
Department of Cellular Biology, Faculty of Medicine, Complutense University, 28040 Madrid, Spain.
Int J Mol Sci. 2023 Jan 4;24(2):960. doi: 10.3390/ijms24020960.
Increased adiposity is related to oxidative stress, inflammation and metabolic disorders. Our group has shown that melatonin totally or partially prevents the alterations that obesity causes in some neuroendocrine and inflammatory parameters indicative of oxidative stress. This study analyzes the effects of HFD on the relative gene expression of several redox balance enzymes on adult male Wistar rats subcutaneous (SAT) and perirenal adipose tissue (PRAT) and the possible preventive role of melatonin. Three experimental groups were established: control, high fat diet (HFD) and HFD plus 25 μg/mL melatonin in tap water. After 11 weeks, animals were sacrificed at 09:00 a.m. and 01:00 a.m. and PRAT and SAT were collected for selected redox enzymes qRT-PCR. Differential expression of redox enzyme genes, except for SOD, GPx and catalase, was observed in the control group as a function of fat depot. HFD causes the disappearance of the temporal changes in the expression of the genes studied in the two fat depots analyzed. PRAT seems to be more sensitive than SAT to increased oxidative stress induced by obesity. Melatonin combined with a HFD intake, partially prevents the effects of the HFD on the gene expression of the redox enzymes. According to our results, melatonin selectively prevents changes in the relative gene expression of redox enzymes in PRAT and SAT of animals fed an HFD.
肥胖与氧化应激、炎症和代谢紊乱有关。我们的研究小组已经表明，褪黑素完全或部分预防了肥胖引起的一些神经内分泌和炎症参数的改变，这些参数表明存在氧化应激。本研究分析了高脂肪饮食（HFD）对成年雄性 Wistar 大鼠皮下（SAT）和肾周脂肪组织（PRAT）中几种氧化还原平衡酶的相对基因表达的影响，以及褪黑素的可能预防作用。建立了三个实验组：对照组、高脂肪饮食组（HFD）和 HFD 加 25μg/ml 褪黑素在自来水中。11 周后，动物在上午 9 点和凌晨 1 点处死，收集 PRAT 和 SAT 用于选定的氧化还原酶 qRT-PCR。除 SOD、GPx 和过氧化氢酶外，对照组中几种氧化还原酶基因的表达随脂肪库而变化。HFD 导致在分析的两个脂肪库中研究的基因表达的时间变化消失。PRAT 似乎比 SAT 对肥胖引起的氧化应激增加更为敏感。褪黑素与 HFD 摄入联合使用，部分预防了 HFD 对氧化还原酶基因表达的影响。根据我们的结果，褪黑素选择性地预防了 HFD 喂养动物的 PRAT 和 SAT 中氧化还原酶相对基因表达的变化。
