Rivière M, Fournié J J, Puzo G
Centre de Recherche de Biochimie et de Génétique Cellulaires, Centre National de la Recherche Scientifique, Toulouse, France.
J Biol Chem. 1987 Nov 5;262(31):14879-84.
Using high-performance liquid chromatography, a new kind of phenolic glycolipid quantitatively minor, called phenolic glycolipid-II, was isolated from a lipidic fraction of Mycobacterium kansasii. The structure was determined by fast atom bombardment-mass spectrometry and proton nuclear magnetic resonance spectroscopy, as: 2,4-di-O-Me-alpha-D-Manp(1----3) 4-O-Ac-2-O-Me-alpha-L-Fucp(1----3)2-O-Me- alpha-L-Rhap(1----3) 2,4-di-O-Me-alpha-L-Rhap 1----phenolphthiocerol dimycocerosate. Phenolic glycolipids I and II differ only by their distal monosaccharide hapten which is 2,6-dideoxy-4-O-Me-alpha-D-arabinohexopyranosyl and the 2,4-di-O-Me-alpha-D-mannopyranosyl, respectively. This sugar appears to be characteristic and apparently unique in the Mycobacterium genus. Moreover, phenolic glycolipids I and II constitute with the lipooligosaccharides two classes of antigens of M. kansasii.
使用高效液相色谱法，从堪萨斯分枝杆菌的脂质部分中分离出一种新的、定量上较少的酚糖脂，称为酚糖脂-II。其结构通过快原子轰击质谱法和质子核磁共振光谱法确定为：2,4-二-O-甲基-α-D-甘露糖基(1→3) 4-O-乙酰基-2-O-甲基-α-L-岩藻糖基(1→3) 2-O-甲基-α-L-鼠李糖基(1→3) 2,4-二-O-甲基-α-L-鼠李糖基 1→酚基硫脂二霉菌酸酯。酚糖脂I和II的区别仅在于其末端单糖半抗原，分别为2,6-二脱氧-4-O-甲基-α-D-阿拉伯己糖基和2,4-二-O-甲基-α-D-甘露糖基。这种糖似乎是分枝杆菌属中的特征性且明显独特的糖。此外，酚糖脂I和II与脂寡糖一起构成了堪萨斯分枝杆菌的两类抗原。
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