Zhu Chunnan, Zhang Jingfang, Zhang Shunrun, Liu Chao, Liu Xiaojun, Jin Jian, Zheng Dongyun
College of Biomedical Engineering, South-Central Minzu University, Wuhan 430074, China.
Key Laboratory of Brain Cognitive Science (State Ethnic Affairs Commission), South-Central Minzu University, Wuhan 430074, China.
Micromachines (Basel). 2023 Jan 13;14(1):198. doi: 10.3390/mi14010198.
A novel nanocomposite film composed of gold nanoparticles and acetylene black-dihexadecyl phosphate was fabricated and modified on the surface of a glassy carbon electrode through a simple and controllable dropping and electropolymerization method. The nanocomposite film electrode showed a good electrocatalytic response to the oxidation of homocysteine and can work as an amperometric biomedical sensor for homocysteine. With the aid of scanning electron microscopy, energy dispersive X-ray technology and electrochemical impedance spectroscopy, the sensing interface was characterized, and the sensing mechanism was discussed. Under optimal conditions, the oxidation peak current of homocysteine was linearly increased with its concentration in the range of 3.0 µmol/L~1.0 mmol/L, and a sensitivity of 18 nA/(μmol/L) was obtained. Furthermore, the detection limit was determined as 0.6 µmol/L, and the response time was detected as 3 s. Applying the nanocomposite film electrode for monitoring the homocysteine in human blood serum, the results were satisfactory.
通过一种简单且可控的滴涂和电聚合方法，制备了一种由金纳米颗粒和乙炔黑 - 二磷酸十六烷基酯组成的新型纳米复合膜，并将其修饰在玻碳电极表面。该纳米复合膜电极对同型半胱氨酸的氧化表现出良好的电催化响应，可作为同型半胱氨酸的安培型生物医学传感器。借助扫描电子显微镜、能量色散X射线技术和电化学阻抗谱对传感界面进行了表征，并讨论了传感机制。在最佳条件下，同型半胱氨酸的氧化峰电流在3.0 μmol/L至1.0 mmol/L范围内随其浓度线性增加，灵敏度为18 nA/(μmol/L)。此外，检测限确定为0.6 μmol/L，响应时间检测为3 s。将该纳米复合膜电极应用于监测人体血清中的同型半胱氨酸，结果令人满意。
