Frantsuzova Ekaterina, Delegan Yanina, Bogun Alexander, Sokolova Diyana, Nazina Tamara
Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of Russian Academy of Sciences" (FRC PSCBR RAS), 142290 Pushchino, Russia.
State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia.
Microorganisms. 2022 Dec 20;11(1):4. doi: 10.3390/microorganisms11010004.
A number of actinobacteria of the genus are able to use dibenzothiophene (DBT) and its derivatives as the only source of sulfur, which makes them promising agents for the process of oil biodesulfurization. Actinobacteria assimilate sulfur from condensed thiophenes without breaking the carbon-carbon bonds, using the 4S pathway encoded by the dszABC operon-like structure. The genome of the new dibenzothiophene-degrading hydrocarbon-oxidizing bacterial strain 6-1 was completely sequenced and the genes potentially involved in the pathways of DBT desulfurization, oxidation of alkanes and aromatic compounds, as well as in the osmoprotectant metabolism in strain 6-1 and other members of the genus , were analyzed. The genome of . strain 6-1 consists of a 5,105,798-bp circular chromosome (67.3% GC content) and an 86,621-bp circular plasmid, pCP86 (65.4% GC content). This paper presents a comparative bioinformatic analysis of complete genomes of strain 6-1 and dibenzothiophene-degrading strains 1D and 135 that do not have the dsz operon. The assumption is made about the participation in this process of the region containing the B gene. Genomic analysis supported the results of phenomenological studies of strains and the possibility of their application in the bioremediation of oil-contaminated environments and in the purification of oil equipment from oil and asphalt-resin-paraffin deposits.
多个属的放线菌能够将二苯并噻吩（DBT）及其衍生物作为唯一的硫源，这使其成为石油生物脱硫过程中有前景的试剂。放线菌通过由dszABC操纵子样结构编码的4S途径，在不破坏碳 - 碳键的情况下从稠合噻吩中同化硫。对新的二苯并噻吩降解烃氧化细菌菌株6 - 1的基因组进行了全序列测定，并分析了可能参与DBT脱硫途径、烷烃和芳香化合物氧化途径以及菌株6 - 1和该属其他成员中渗透保护剂代谢的基因。菌株6 - 1的基因组由一个5,105,798 bp的环状染色体（GC含量为67.3%）和一个86,621 bp的环状质粒pCP86（GC含量为65.4%）组成。本文对菌株6 - 1以及不含dsz操纵子的二苯并噻吩降解菌株1D和135的完整基因组进行了比较生物信息学分析。对含有B基因的区域参与该过程进行了假设。基因组分析支持了对菌株的现象学研究结果以及它们在石油污染环境生物修复和从石油及沥青 - 树脂 - 石蜡沉积物中净化石油设备方面应用的可能性。
