酸水解后多西拉敏人工代谢产物的形成。

Formation of artifactual metabolites of doxylamine following acid hydrolysis.

作者信息

Holder C L, Korfmacher W A, Rushing L G, Thompson H C, Slikker W, Gosnell A B

机构信息

Department of Health and Human Services, Food and Drug Administration, Jefferson, AR 72079.

出版信息

J Chromatogr. 1987 Aug 7;419:113-22. doi: 10.1016/0378-4347(87)80271-2.

DOI:10.1016/0378-4347(87)80271-2

PMID:3667771

Abstract

This study describes the use of gas chromatographic-mass spectrometric, high-performance liquid chromatographic and capillary column gas chromatographic separation techniques in demonstrating the production of several artifactual compounds reported in the literature as metabolites of doxylamine. Rhesus monkey urinary extracts which contained doxylamine and doxylamine metabolites were examined with and without acid hydrolysis. The production of 1-phenyl-1-(2-pyridinyl)ethanol and 1-phenyl-1-(2-pyridinyl)ethylene under acid hydrolysis conditions was demonstrated. These artifactual products were shown to originate from the acid hydrolysis of 2-[1-phenyl-1-(2-pyridinyl)ethoxy] acetic acid and not from doxylamine.

摘要

本研究描述了使用气相色谱 - 质谱联用、高效液相色谱和毛细管柱气相色谱分离技术，来证明文献中报道的作为多西拉敏代谢物的几种人为产物的产生。对含有多西拉敏和多西拉敏代谢物的恒河猴尿液提取物进行了酸水解和未酸水解的检测。结果表明在酸水解条件下会产生1 - 苯基 - 1 -（2 - 吡啶基）乙醇和1 - 苯基 - 1 -（2 - 吡啶基）乙烯。这些人为产物被证明源自2 - [1 - 苯基 - 1 -（2 - 吡啶基）乙氧基]乙酸的酸水解，而非多西拉敏。