de Jong A P, Wittebrood A J, du Châtinier W M, Bron J
Cedona Pharmaceuticals B.V., Haarlem, The Netherlands.
J Chromatogr. 1987 Aug 7;419:233-42. doi: 10.1016/0378-4347(87)80281-5.
A universal rapid, sensitive and selective high-performance liquid chromatographic method with UV detection at 230 nm has been developed for the determination of benzamide drugs in human plasma and urine. Sample pretreatment is carried out using solid-phase extraction columns, resulting in very high extraction recoveries of the compounds investigated (alizapride, metoclopramide, alpiropride, amisulpride). The detector response is linear from 25 to 10,000 ng/ml, and the detection limit is 3 ng/ml for alizapride and 10 ng/ml for metoclopramide. The proposed method is highly suitable for pharmacokinetic studies and for drug monitoring.
已开发出一种通用的快速、灵敏且选择性高的高效液相色谱法，用于在230 nm波长下进行紫外检测，以测定人血浆和尿液中的苯甲酰胺类药物。使用固相萃取柱进行样品预处理，所研究的化合物（阿立必利、甲氧氯普胺、阿哌立醇、氨磺必利）的提取回收率非常高。检测器响应在25至10,000 ng/ml范围内呈线性，阿立必利的检测限为3 ng/ml，甲氧氯普胺的检测限为10 ng/ml。该方法非常适合药代动力学研究和药物监测。
