Department of Pharmacy, Changhai Hospital, Naval Medical University (Second Military Medical University), Shanghai 200433, China.
Department of Gastroenterology, Changzheng Hospital, Naval Medical University (Second Military Medical University), Shanghai 200433, China.
Molecules. 2023 Jan 16;28(2):880. doi: 10.3390/molecules28020880.
Over the past decade, the dose of nanoparticles given to solid tumors has remained at a median of 0.7% of the injected dose. Most nanoparticles are trapped in a mononuclear phagocyte system (MPS), of which 85% are Kupffer cells. In our study, threshold doses of bovine serum albumin (BSA) nanoparticles were investigated for the uptake of Kupffer cells in vitro and in vivo. The antitumor effect and safety of albumin-bound paclitaxel (ABP) were improved by using threshold doses of BSA nanoparticles. We found a threshold dose of 20,000 nanoparticles per macrophage uptake in vitro and a saturation dose of 0.3 trillion nanoparticles in tumor-bearing mice. In vivo efficacy and safety evaluations demonstrated that the threshold doses of blank BSA nanoparticles could significantly improve the efficacy and safety of ABP against tumors compared with ABP alone. In this study, the delivery efficiency of ABP was improved by using blank nanoparticles to saturate Kupffer cells, which provided a new approach to studying the Kupffer cell saturation threshold and thus a new scheme for improving the curative effect of ABP.
在过去的十年中，给予实体瘤的纳米颗粒剂量中位数一直保持在注射剂量的 0.7%。大多数纳米颗粒被单核吞噬细胞系统 (MPS) 捕获，其中 85%是库普弗细胞。在我们的研究中，研究了牛血清白蛋白 (BSA) 纳米颗粒的阈值剂量，以研究其在体外和体内对库普弗细胞的摄取。使用 BSA 纳米颗粒的阈值剂量可以提高白蛋白结合紫杉醇 (ABP) 的抗肿瘤作用和安全性。我们发现，体外每吞噬细胞摄取 20000 个纳米颗粒为阈值剂量，荷瘤小鼠的饱和剂量为 0.3 万亿个纳米颗粒。体内疗效和安全性评估表明，与单独使用 ABP 相比，空白 BSA 纳米颗粒的阈值剂量可显著提高 ABP 对肿瘤的疗效和安全性。在这项研究中，通过使用空白纳米颗粒使库普弗细胞饱和来提高 ABP 的递送效率，为研究库普弗细胞饱和阈值提供了一种新方法，从而为提高 ABP 的治疗效果提供了一种新方案。
