Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China.
Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Jiangxi Agricultural University, Nanchang 330045, China.
Nutrients. 2023 Jan 14;15(2):435. doi: 10.3390/nu15020435.
is an economically important oilseed crop and medical plant. However, as a characteristic honey resource, the standard protocol used to identify the composition of honey has not been established yet. Previously, distinctive flavonoid has been shown as an effective marker to trace the botanical origin of honey. In this study, we examined the flavonoid types in honey and nine other monofloral honeys by using liquid chromatography tandem-mass spectrometry (LC-MS/MS) and compared the differences and identified eight distinct flavonoids in honey. Then, comparing the 8 flavonoids with the 14 flavonoids common to honey and nectar, two distinct flavonoids were identified in honey and nectar. Finally, we identified kaempferitrin as the distinct flavonoid marker in honey using the degree of influence of the partial least-squares discriminant analysis (PLS-DA) model on honey and ployfloral honey.
是一种具有重要经济价值的油料作物和药用植物。然而，作为一种特殊的蜜源，尚未建立用于鉴定蜂蜜成分的标准方法。此前，已证明独特的类黄酮是追踪蜂蜜植物来源的有效标志物。在这项研究中，我们使用液相色谱串联质谱（LC-MS/MS）检测了 蜂蜜和其他 9 种单花蜜中的类黄酮类型，并比较了它们之间的差异，在 蜂蜜中鉴定出了 8 种不同的类黄酮。然后，将这 8 种类黄酮与 蜂蜜和花蜜中共有的 14 种类黄酮进行比较，在 蜂蜜和花蜜中鉴定出了两种独特的类黄酮。最后，我们使用偏最小二乘判别分析（PLS-DA）模型对 蜂蜜和多花蜜的影响程度，鉴定出了 kaempferitrin 是 蜂蜜中的独特类黄酮标志物。
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