Research Group on Quality, Safety and Bioactivity of Plant Foods, CEBAS-CSIC, P.O. Box 164, Espinardo 30100, Murcia, Spain.
J Chromatogr A. 2009 Oct 23;1216(43):7241-8. doi: 10.1016/j.chroma.2009.07.057. Epub 2009 Aug 3.
HPLC-MS-MS analysis of unifloral honey extracts has shown the occurrence of flavonoid glycosides in most of the analyzed samples. These compounds are not present in large amounts, but can reach up to 600 microg/100g honey in canola and rapeseed honeys. Rhamnosyl-hexosides (tentatively rutinosides and neohesperidosides) and dihexosides (hexosyl(1-->2)hexosides and hexosyl(1-->6)hexosides) of flavonols such as quercetin, kaempferol, isorhamnetin and 8-methoxykaempferol, are the main flavonoid glycosides found in honey. However, flavonoid triglycosides and monoglycosides are also detected in some floral origins. Eucalyptus and orange blossom nectars were collected and analyzed showing that nectar flavonoid glucosides, as is the case of eucalyptus flavonoids, can be readily hydrolyzed by the bee saliva enzymes, while flavonoid rhamnosyl-glucosides, as is the case of citrus nectar flavonoids, are not hydrolyzed, and because of these reasons the flavonoid glycoside content of citrus honey is higher than that of eucalyptus honey that contains mainly aglycones. The flavonoid glycoside profiles detected in honeys suggest that this could be related to their floral origin and the results show that the HPLC-MSn ion trap analysis of flavonoid glycosides in honey is a promising analytical method to help in the objective determination of the floral origin of unifloral honeys.
利用高效液相色谱-串联质谱法（HPLC-MS-MS）对单一花源蜂蜜提取物进行分析的结果表明，在所分析的大部分样品中都存在类黄酮糖苷。这些化合物的含量并不高，但在油菜和芥菜蜂蜜中可达到 600 微克/100 克蜂蜜。在蜂蜜中发现的主要类黄酮糖苷为黄酮醇的鼠李糖苷-己糖苷（暂定芦丁糖苷和新橙皮糖苷）和二己糖苷（己糖基（1--2）己糖苷和己糖基（1--6）己糖苷），如槲皮素、山奈酚、异鼠李素和 8-甲氧基山奈酚。然而，在某些花源中也检测到类黄酮三糖苷和单糖苷。采集并分析了桉树和橙花蜜，结果表明，像桉树黄酮一样，花蜜类黄酮葡萄糖苷可以被蜜蜂唾液酶迅速水解，而像柑橘花蜜类黄酮一样，类黄酮鼠李糖苷则不会被水解，因此柑橘蜂蜜中的类黄酮糖苷含量高于主要含有苷元的桉树蜂蜜。蜂蜜中检测到的类黄酮糖苷图谱表明，这可能与其花源有关，并且结果表明，利用高效液相色谱-串联质谱-离子阱分析蜂蜜中的类黄酮糖苷是一种很有前途的分析方法，可以帮助客观确定单一花源蜂蜜的花源。
