Matsushita M, Ikeda M
Department of Anatomy, University of Tsukuba, Ibaraki, Japan.
J Comp Neurol. 1987 Sep 8;263(2):223-40. doi: 10.1002/cne.902630206.
The projection fields of spinocerebellar tracts arising from the cervical enlargement were studied by the anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) in the cat. Following injections of WGA-HRP into the C5-C8 or T1 segments, labeled terminals were seen in lobule I to sublobule Vf of the anterior lobe, and lobule VI, sublobule VIIb, lobules VIII and IX, the simple lobule, crus II, and the paramedian lobule of the posterior lobe. In the sagittal plane of sublobules Ib-Vf and sublobules VIf and VId, the labeled terminals were distributed mainly in the superficial two thirds of the apicobasal extent. The labeled terminals in the anterior lobe accounted for about 70% of the total labeled terminals; the great majority were in lobule IV (17-20%) and lobule V (40%). The labeled terminals in the posterior lobe accounted for 30% of the total labeled terminals; the majority were in sublobules VIf (11-15%) and VId (6%). In the mediolateral extent, more than 50% of the total labeled terminals in each lobule were concentrated within 1.0 mm from the midline (the vermis) and 70-80% within 2.0 mm from the midline (the vermis and the medial part of the intermediate regions). A smaller number were also present in the intermediate region. Cases with lateral cordotomies revealed that the projections were bilateral but predominantly ipsilateral to the cells or origin and that the quantity of the ipsilateral projection was 66.5-75% of the total in each of sublobules IVb, Va, and VIf. The projection to the paramedian lobule was also predominantly ipsilateral. Projection fields in the horizontal plane were reconstructed from a series of transverse sections through each lobule. In sublobules Va-VIf labeled terminals were distributed in three areas: area 1 located within 0.25 mm from the midline in zone A1 of Voogd; area 2 located between 0.5 and 0.75 mm lateral to the midline in zones A1 and A2; and area 3, which appeared to be located between 0.75 and 1.5 mm lateral to the midline in zones A2-B. These areas extended longitudinally in the apical two thirds of the lobules. From the present and previous retrograde HRP studies it was suggested that the neuronal groups in the cervical enlargement (the medial lamina VI group and the central lamina VII group) project to lobules I-V of the anterior lobe and lobule VI, sublobule VIIb, and lobule VIII of the posterior lobe. They have strong projections to caudal lobules of the anterior lobe (lobules III-Va) and lobules facing the primary fissure (sublobules Vd-Vf and sublobules VIf and VId).
通过将与辣根过氧化物酶结合的小麦胚芽凝集素（WGA-HRP）进行顺行运输，在猫身上研究了颈膨大产生的脊髓小脑束的投射区域。将WGA-HRP注入C5-C8或T1节段后，在前叶的I小叶至Vf小叶、VI小叶、VIIb小叶、VIII和IX小叶、单小叶、II脚以及后叶的旁正中小叶中可见标记终末。在Ib-Vf小叶和VIf及VId小叶的矢状平面上，标记终末主要分布在顶-基范围的浅表三分之二处。前叶中的标记终末约占总标记终末的70%；绝大多数位于IV小叶（17%-20%）和V小叶（40%）。后叶中的标记终末占总标记终末的30%；大多数位于VIf小叶（11%-15%）和VId小叶（6%）。在内外侧范围内，每个小叶中超过50%的总标记终末集中在距中线（蚓部）1.0mm范围内，70%-80%集中在距中线（蚓部和中间区内侧部分）2.0mm范围内。中间区也有少量标记终末。侧索切断的病例显示，投射是双侧的，但主要是同侧于细胞或起源，并且同侧投射的数量在IVb、Va和VIf每个小叶中占总数的66.5%-75%。向旁正中小叶的投射也主要是同侧的。通过一系列穿过每个小叶的横切面重建了水平面的投射区域。在Va-VIf小叶中，标记终末分布在三个区域：区域1位于Voogd的A1区距中线0.25mm范围内；区域2位于A1和A2区距中线外侧0.5至0.75mm之间；区域3似乎位于A2-B区距中线外侧0.75至1.5mm之间。这些区域在小叶的顶三分之二处纵向延伸。根据目前和以前的逆行HRP研究表明，颈膨大（内侧VI层组和中央VII层组）中的神经元群投射到前叶的I-V小叶以及后叶的VI小叶、VIIb小叶和VIII小叶。它们对前叶的尾侧小叶（III-Va小叶）和面对初级裂的小叶（Vd-Vf小叶和VIf及VId小叶）有强投射。
