Department of Biological and Chemical Engineering, Aarhus University, Denmark; Sino-Danish College (SDC), University of Chinese Academy of Sciences, Beijing 101400, China.
Department of Biological and Chemical Engineering, Aarhus University, Denmark.
Biomater Adv. 2023 Mar;146:213284. doi: 10.1016/j.bioadv.2023.213284. Epub 2023 Jan 7.
Major challenges in developing implanted neural stimulation devices are the invasiveness, complexity, and cost of the implantation procedure. Here, we report an injectable, nanofibrous 2D flexible hydrogel sheet-based neural stimulation device that can be non-invasively implanted via syringe injection for optoelectrical and biochemical dual stimulation of neuron. Specifically, methacrylated gelatin (GelMA)/alginate hydrogel nanofibers were mechanically reinforced with a poly(lactide-co-ε-caprolactone) (PLCL) core by coaxial electrospinning. The lubricant hydrogel shell enabled not only injectability, but also facile incorporation of functional nanomaterials and bioactives. The nanofibers loaded with photocatatlytic g-CN/GO nanoparticles were capable of stimulating neural cells via blue light, with a significant 36.3 % enhancement in neurite extension. Meanwhile, the nerve growth factor (NGF) loaded nanofibers supported a sustained release of NGF with well-maintained function to biochemically stimulate neural differentiation. We have demonstrated the capability of an injectable, hydrogel nanofibrous, neural stimulation system to support neural stimulation both optoelectrically and biochemically, which represents crucial early steps in a larger effort to create a minimally invasive system for neural stimulation.
开发植入式神经刺激设备的主要挑战在于植入手术的侵入性、复杂性和成本。在这里，我们报告了一种可注射的、基于纳米纤维的 2D 柔性水凝胶片状神经刺激装置，它可以通过注射器注射进行非侵入式植入，用于光电和生化双重刺激神经元。具体来说，通过同轴静电纺丝，将甲基丙烯酰化明胶（GelMA）/海藻酸钠水凝胶纳米纤维用聚（乳酸-共-ε-己内酯）（PLCL）芯进行机械增强。润滑水凝胶壳不仅使可注射性成为可能，而且还便于掺入功能性纳米材料和生物活性物质。负载光催化剂 g-CN/GO 纳米粒子的纳米纤维能够通过蓝光刺激神经细胞，使神经突延伸率显著提高 36.3%。同时，负载神经生长因子（NGF）的纳米纤维支持 NGF 的持续释放，同时保持其功能，以生化方式刺激神经分化。我们已经证明了可注射的水凝胶纳米纤维神经刺激系统具有光电和生化双重刺激神经的能力，这代表了创建微创神经刺激系统的更大努力中的关键早期步骤。
