Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju Special Self-Governing Province, 63243, Korea.
Stem Cell Research Center, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju Special Self-Governing Province, 63243, Korea.
Zygote. 2023 Feb;31(1):14-24. doi: 10.1017/S0967199422000399.
This study investigated the effect of the flavonoid-based compound isorhamnetin (ISO) on maturation and developmental competence in oxidative stress-exposed porcine oocytes . Treatment with 2 μM ISO (2 ISO) increases the developmental rate of oxidative stress-exposed porcine oocytes during maturation (IVM). The glutathione level and mRNA expression of antioxidant-related genes ( and ) were increased in the 2 ISO-treated group, whereas the reactive oxygen species level was decreased. Treatment with 2 ISO increased mRNA expression of a cumulus cell expansion-related gene () and improved chromosomal alignment. mRNA expression of maternal genes ( and ) and mitogen activated protein kinase (MAPK) activity were increased in the 2 ISO-treated group. The total cell number per blastocyst and percentage of apoptotic cells were increased and decreased in the 2 ISO-treated group, respectively. Treatment with 2 ISO increased mRNA expression of development-related genes (, and ) and anti-apoptotic genes ( and ) and decreased that of pro-apoptotic genes ( and ). These results demonstrate that 2 ISO improves the quality of porcine oocytes by protecting them against oxidative stress during IVM and enhances subsequent embryo development . Therefore, we propose that ISO is a useful supplement for IVM of porcine oocytes.
本研究探讨了基于类黄酮的化合物异鼠李素（ISO）对暴露于氧化应激的猪卵母细胞成熟和发育能力的影响。用 2 μM ISO（2 ISO）处理可提高氧化应激暴露的猪卵母细胞在成熟（IVM）过程中的发育率。与对照组相比，2 ISO 处理组的谷胱甘肽水平和抗氧化相关基因（和）的 mRNA 表达增加，而活性氧水平降低。2 ISO 处理增加了与卵丘细胞扩展相关的基因（）的 mRNA 表达，并改善了染色体排列。2 ISO 处理组母性基因（和）和丝裂原激活蛋白激酶（MAPK）活性的 mRNA 表达增加。2 ISO 处理组每个囊胚的总细胞数增加，凋亡细胞的百分比减少。2 ISO 处理增加了与发育相关的基因（、和）和抗凋亡基因（和）的 mRNA 表达，降低了促凋亡基因（和）的表达。这些结果表明，2 ISO 通过在 IVM 期间保护猪卵母细胞免受氧化应激，从而提高卵母细胞的质量，并增强随后的胚胎发育。因此，我们提出 ISO 是猪卵母细胞 IVM 的有用补充。
