Gao Jianping, Guo Zhenhu, Zhang Yang, Liu Yuying, Xing Fangyu, Wang Junjie, Luo Xi, Kong Yingjun, Zhang Guifeng
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
Regen Biomater. 2022 Dec 29;10:rbac110. doi: 10.1093/rb/rbac110. eCollection 2023.
The content of type I collagen (COL-I) and type III collagen (COL-III) and the ratio between them not only affect the skin elasticity and mechanical strength, but also determine the fibril diameter. In this research, we investigated the age-related changes in COL-I/COL-III ratio with their formed fibril diameter. The experimental result was obtained from high performance liquid chromatography-mass spectrometer, hydroxyproline determination, picrosirius red staining and transmission electron microscopes (TEM), respectively. The result indicated that the COL-I/COL-III ratio in mouse skin increased with aging. From the 0th to 9th week, the COL-I/COLIII ratio increased from 1.3:1 to 4.5:1. From the 9th to the 18th week, it remained between 4.5:1 and 4.9:1. The total content of COL-I and COL-III firstly increased and then decreased with aging. The TEM result showed that the fibril diameter increased with aging. From the 0th to 9th week, the average fibril diameter increased from 40 to 112 nm; From the 9th to 18th weeks, it increased from 112 to 140 nm. After the 9th week. The fibril diameter showed obvious uneven distribution. Thus, the COL-I/COLIII ratio was proportional to the fibril diameter, but inversely proportional to the uniformity of fibril diameter.
I型胶原蛋白（COL-I）和III型胶原蛋白（COL-III）的含量及其比例不仅影响皮肤弹性和机械强度，还决定原纤维直径。在本研究中，我们调查了COL-I/COL-III比例随年龄的变化及其形成的原纤维直径。实验结果分别来自高效液相色谱-质谱仪、羟脯氨酸测定、天狼星红染色和透射电子显微镜（TEM）。结果表明，小鼠皮肤中的COL-I/COL-III比例随年龄增长而增加。从第0周到第9周，COL-I/COLIII比例从1.3:1增加到4.5:1。从第9周到第18周，它保持在4.5:1和4.9:1之间。COL-I和COL-III的总含量随年龄增长先增加后减少。TEM结果表明，原纤维直径随年龄增长而增加。从第0周到第9周，平均原纤维直径从40增加到112nm；从第9周到第18周，它从112增加到140nm。在第9周之后。原纤维直径显示出明显的不均匀分布。因此，COL-I/COLIII比例与原纤维直径成正比，但与原纤维直径的均匀性成反比。
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