Montgomery G W, Martin G B, Locatelli A, Pelletier J
INRA, Station de Physiologie de la Reproduction, Nouzilly, France.
J Endocrinol. 1987 Sep;114(3):437-42. doi: 10.1677/joe.0.1140437.
An experiment was conducted to measure LH pulse frequencies in bilaterally adrenalectomized Ile-de-France ewes during the mid-anoestrous season. Seven ewes were adrenalectomized under general anaesthesia and maintained on daily injections of 3 mg deoxycorticosterone acetate (DOCA). Adrenalectomy did not affect the patterns of LH release and the mean intervals between pulses in the adrenalectomized and sham-operated control ewes were 48 and 52 min respectively. Small implants of oestradiol significantly reduced the frequency of LH pulses in both groups and, in the presence of oestradiol, there were no differences in LH release between adrenalectomized and sham-operated ewes, with the mean interpulse intervals being 91 and 89 min respectively. In a second experiment, designed to assess the effects of DOCA on LH release, the mean interpulse intervals in unoperated control ewes (46 min) and unoperated ewes given daily injections of 3 mg DOCA (47 min) were similar to those observed in adrenalectomized and sham-operated ewes. In the presence of small implants of oestradiol, the combination of DOCA and oestradiol decreased the pulse frequency significantly more than oestradiol alone (mean interpulse intervals of 85 and 65 min respectively) suggesting that DOCA may act as a progestagen in sheep. Thus alternative treatments to DOCA for the maintenance of adrenalectomized sheep must be found for future studies on the role of adrenal steroids in the reproductive system. It appears that adrenal steroids do not play a major role in the seasonal changes in LH release in the ewe.
进行了一项实验，以测量双侧肾上腺切除的法兰西岛母羊在发情中期的促黄体生成素（LH）脉冲频率。七只母羊在全身麻醉下进行肾上腺切除术，并每天注射3毫克醋酸脱氧皮质酮（DOCA）以维持。肾上腺切除术并未影响LH释放模式，肾上腺切除的母羊和假手术对照母羊的脉冲平均间隔分别为48分钟和52分钟。小剂量植入雌二醇显著降低了两组的LH脉冲频率，并且在存在雌二醇的情况下，肾上腺切除的母羊和假手术母羊之间的LH释放没有差异，脉冲平均间隔分别为91分钟和89分钟。在第二个实验中，旨在评估DOCA对LH释放的影响，未手术的对照母羊（46分钟）和每天注射3毫克DOCA的未手术母羊（47分钟）的脉冲平均间隔与肾上腺切除和假手术母羊中观察到的相似。在存在小剂量植入雌二醇的情况下，DOCA和雌二醇的组合比单独使用雌二醇更显著地降低了脉冲频率（脉冲平均间隔分别为85分钟和65分钟），这表明DOCA在绵羊中可能起孕激素的作用。因此，为了未来关于肾上腺类固醇在生殖系统中作用的研究，必须找到替代DOCA来维持肾上腺切除母羊的方法。肾上腺类固醇似乎在母羊LH释放的季节性变化中不发挥主要作用。
