Norstedt G, Möller C
Karolinska Institute, Centre for Biotechnology, Huddinge, Sweden.
J Endocrinol. 1987 Oct;115(1):135-9. doi: 10.1677/joe.0.1150135.
Primary liver cells from adult rats were used to study the hormonal regulation of mRNA coding for insulin-like growth factor I (IGF-I). IGF-I mRNA could be detected at a low concentration in liver cells prepared from hypophysectomized rats and cultivated for 3 days. When these cells were exposed to GH on the second day, a dose-dependent increase in IGF-I mRNA was observed. The continuous presence of serum was not a prerequisite for this response, since GH also induced IGF-I mRNA in a defined hormone-supplemented medium. It is concluded that GH can induced IGF-I mRNA in the liver by a direct mechanism.
采用成年大鼠的原代肝细胞来研究胰岛素样生长因子I（IGF-I）编码mRNA的激素调节。在取自垂体切除大鼠并培养3天的肝细胞中，可检测到低浓度的IGF-I mRNA。当这些细胞在第二天暴露于生长激素（GH）时，观察到IGF-I mRNA呈剂量依赖性增加。血清的持续存在并非这种反应的先决条件，因为在特定的激素补充培养基中，GH也能诱导IGF-I mRNA。得出的结论是，GH可通过直接机制诱导肝脏中的IGF-I mRNA。
