Chen Liuzhu, Duan Liu, Sun Minghui, Yang Zhuo, Li Hongyu, Hu Keming, Yang Hong, Liu Li
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China.
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou, China.
Front Plant Sci. 2023 Jan 4;13:1052569. doi: 10.3389/fpls.2022.1052569. eCollection 2022.
Ethyl methanesulfonate (EMS)-induced mutagenesis is a powerful tool to generate genetic resource for identifying untapped genes and characterizing the function of genes to understand the molecular basis of important agronomic traits. This review focuses on application of contemporary EMS mutagenesis in the field of plant development and abiotic stress tolerance research, with particular focuses on reviewing the mutation types, mutagenesis site, mutagen concentration, mutagenesis duration, the identification and characterization of mutations responsible for altered stress tolerance responses. The application of EMS mutation breeding combined with genetic engineering in the future plant breeding and fundamental research was also discussed. The collective information in this review will provide good insight on how EMS mutagenesis is efficiently applied to improve abiotic stress tolerance of crops with the utilization of Next-generation sequencing (NGS) for mutation identification.
甲基磺酸乙酯（EMS）诱变是一种强大的工具，可用于生成遗传资源，以识别未开发的基因并表征基因功能，从而了解重要农艺性状的分子基础。本综述重点关注当代EMS诱变在植物发育和非生物胁迫耐受性研究领域的应用，特别着重于回顾突变类型、诱变位点、诱变剂浓度、诱变持续时间，以及对负责改变胁迫耐受性反应的突变的鉴定和表征。还讨论了EMS突变育种与基因工程相结合在未来植物育种和基础研究中的应用。本综述中的汇总信息将为如何有效应用EMS诱变，利用下一代测序（NGS）进行突变鉴定来提高作物的非生物胁迫耐受性提供很好的见解。
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