• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

优化菌株和发酵工艺以提高L-赖氨酸产量：综述

Optimizing strains and fermentation processes for enhanced L-lysine production: a review.

作者信息

Wu Zijuan, Chen Tianpeng, Sun Wenjun, Chen Yong, Ying Hanjie

机构信息

National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.

出版信息

Front Microbiol. 2024 Oct 4;15:1485624. doi: 10.3389/fmicb.2024.1485624. eCollection 2024.

DOI:10.3389/fmicb.2024.1485624
PMID:39430105
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11486702/
Abstract

lysine is an essential amino acid with significant importance, widely used in the food, feed, and pharmaceutical industries. To meet the increasing demand, microbial fermentation has emerged as an effective and sustainable method for L-lysine production. has become one of the primary microorganisms for industrial L-lysine production due to its rapid growth, ease of genetic manipulation, and high production efficiency. This paper reviews the recent advances in strain engineering and fermentation process optimization for L-lysine production. Additionally, it discusses potential technological breakthroughs and challenges in -based L-lysine production, offering directions for future research to support industrial-scale production.

摘要

赖氨酸是一种具有重要意义的必需氨基酸，广泛应用于食品、饲料和制药行业。为满足不断增长的需求，微生物发酵已成为生产L-赖氨酸的一种有效且可持续的方法。由于其生长迅速、易于进行基因操作且生产效率高，已成为工业生产L-赖氨酸的主要微生物之一。本文综述了用于L-赖氨酸生产的菌株工程和发酵工艺优化的最新进展。此外，还讨论了基于该菌株的L-赖氨酸生产中潜在的技术突破和挑战，为支持工业规模生产的未来研究提供了方向。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/f3864b0f44ed/fmicb-15-1485624-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/c2e40716a83f/fmicb-15-1485624-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/96f6f80d30b3/fmicb-15-1485624-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/63d445ff8ca3/fmicb-15-1485624-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/f3864b0f44ed/fmicb-15-1485624-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/c2e40716a83f/fmicb-15-1485624-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/96f6f80d30b3/fmicb-15-1485624-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/63d445ff8ca3/fmicb-15-1485624-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a5/11486702/f3864b0f44ed/fmicb-15-1485624-g004.jpg

相似文献

1
Optimizing strains and fermentation processes for enhanced L-lysine production: a review.优化菌株和发酵工艺以提高L-赖氨酸产量：综述
Front Microbiol. 2024 Oct 4;15:1485624. doi: 10.3389/fmicb.2024.1485624. eCollection 2024.
2
Industrial production of L-lysine in Corynebacterium glutamicum: Progress and prospects.谷氨酸棒杆菌中L-赖氨酸的工业化生产：进展与展望
Microbiol Res. 2022 Sep;262:127101. doi: 10.1016/j.micres.2022.127101. Epub 2022 Jun 25.
3
[Metabolic engineering of the substrate utilization pathway in increases L-lysine production].[对底物利用途径进行代谢工程改造以提高L-赖氨酸产量]
Sheng Wu Gong Cheng Xue Bao. 2024 Aug 25;40(8):2513-2527. doi: 10.13345/j.cjb.240049.
4
L-lysine production improvement: a review of the state of the art and patent landscape focusing on strain development and fermentation technologies.赖氨酸生产的改进：聚焦于菌株开发和发酵技术的最新技术和专利现状综述。
Crit Rev Biotechnol. 2019 Dec;39(8):1031-1055. doi: 10.1080/07388551.2019.1663149. Epub 2019 Sep 23.
5
Synthetic acid stress-tolerance modules improve growth robustness and lysine productivity of industrial Escherichia coli in fermentation at low pH.合成酸应激耐受模块可提高低 pH 发酵中工业大肠杆菌的生长鲁棒性和赖氨酸产量。
Microb Cell Fact. 2022 Apr 22;21(1):68. doi: 10.1186/s12934-022-01795-4.
6
Metabolic stress constrains microbial L-cysteine production in Escherichia coli by accelerating transposition through mobile genetic elements.代谢应激通过加速移动遗传元件的转座来限制大肠杆菌中微生物 L-半胱氨酸的生产。
Microb Cell Fact. 2023 Jan 16;22(1):10. doi: 10.1186/s12934-023-02021-5.
7
Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid.谷氨酸棒杆菌的代谢工程改造以提高5-氨基戊酸的产量。
Microb Cell Fact. 2016 Oct 7;15(1):174. doi: 10.1186/s12934-016-0566-8.
8
Lysine Fermentation: History and Genome Breeding.赖氨酸发酵：历史与基因组育种
Adv Biochem Eng Biotechnol. 2017;159:73-102. doi: 10.1007/10_2016_27.
9
Recent advances in the metabolic engineering and physiological opportunities for microbial synthesis of L-aspartic acid family amino acids: A review.微生物合成L-天冬氨酸家族氨基酸的代谢工程与生理机遇的最新进展：综述
Int J Biol Macromol. 2023 Dec 31;253(Pt 3):126916. doi: 10.1016/j.ijbiomac.2023.126916. Epub 2023 Sep 15.
10
Integrated strain engineering and bioprocessing strategies for high-level bio-based production of 3-hydroxyvalerate in Escherichia coli.整合应变工程和生物加工策略，以在大肠杆菌中高水平生物合成 3-羟基丁酸酯。
Appl Microbiol Biotechnol. 2020 Jun;104(12):5259-5272. doi: 10.1007/s00253-020-10580-5. Epub 2020 Apr 14.

引用本文的文献

1
Functional and taxonomic dysbiosis of the supragingival plaque metagenome in Behçet's disease.白塞病龈上菌斑宏基因组的功能和分类失调
J Oral Microbiol. 2025 Aug 29;17(1):2552165. doi: 10.1080/20002297.2025.2552165. eCollection 2025.
2
Isolation and identification of Alcaligenes faecalis W2-3 with high-yield production of dimethyl disulfide.高产二甲基二硫的粪产碱杆菌W2-3的分离与鉴定
Sci Rep. 2025 Jul 1;15(1):20830. doi: 10.1038/s41598-025-04904-6.

本文引用的文献

1
[Metabolic engineering of the substrate utilization pathway in increases L-lysine production].[对底物利用途径进行代谢工程改造以提高L-赖氨酸产量]
Sheng Wu Gong Cheng Xue Bao. 2024 Aug 25;40(8):2513-2527. doi: 10.13345/j.cjb.240049.
2
Metabolic Engineering of High L-Lysine-Producing for de Novo Production of L-Lysine-Derived Compounds.通过代谢工程改造高赖氨酸生产菌用于从头合成赖氨酸衍生化合物。
ACS Synth Biol. 2024 Sep 20;13(9):2948-2959. doi: 10.1021/acssynbio.4c00356. Epub 2024 Aug 19.
3
Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.
磷酸烯醇丙酮酸-草酰乙酸-丙酮酸衍生氨基酸生物合成的代谢工程最新进展。
Molecules. 2024 Jun 18;29(12):2893. doi: 10.3390/molecules29122893.
4
Current trends and possibilities of typical microbial protein production approaches: a review.当前典型微生物蛋白生产方法的趋势和可能性：综述。
Crit Rev Biotechnol. 2024 Dec;44(8):1515-1532. doi: 10.1080/07388551.2024.2332927. Epub 2024 Apr 2.
5
Systems engineering of Escherichia coli for high-level glutarate production from glucose.利用大肠杆菌工程系统从葡萄糖生产高浓度戊二酸。
Nat Commun. 2024 Feb 3;15(1):1032. doi: 10.1038/s41467-024-45448-z.
6
Standardized Iterative Genome Editing Method for Based on CRISPR-Cas9.基于 CRISPR-Cas9 的标准化迭代基因组编辑方法。
ACS Synth Biol. 2024 Feb 16;13(2):613-623. doi: 10.1021/acssynbio.3c00585. Epub 2024 Jan 20.
7
Reconstruction, simulation and analysis of enzyme-constrained metabolic models using GECKO Toolbox 3.0.使用GECKO Toolbox 3.0对酶约束代谢模型进行重建、模拟和分析。
Nat Protoc. 2024 Mar;19(3):629-667. doi: 10.1038/s41596-023-00931-7. Epub 2024 Jan 18.
8
Solid-state fermentation production of L-lysine by Corynebacterium glutamicum (ATCC 13032) using agricultural by-products as substrate.利用农业副产物作为底物，通过谷氨酸棒杆菌（ATCC 13032）进行固态发酵生产 L-赖氨酸。
World J Microbiol Biotechnol. 2023 Nov 24;40(1):20. doi: 10.1007/s11274-023-03822-x.
9
A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies.超声辅助提取（UAE）在生物活性成分提取方面的综合综述：原理、优点、设备和联合技术。
Ultrason Sonochem. 2023 Dec;101:106646. doi: 10.1016/j.ultsonch.2023.106646. Epub 2023 Oct 13.
10
Mass-Activated Droplet Sorting for the Selection of Lysine-Producing .大规模激活液滴分选用于赖氨酸产生菌的筛选
Anal Chem. 2023 Oct 24;95(42):15716-15724. doi: 10.1021/acs.analchem.3c03080. Epub 2023 Oct 11.