Zhao Jie, Sun Lijing, Gao Huimin, Hu Mengyun, Mu Liming, Cheng Xiaohu, Wang Jianbing, Zhao Yun, Li Qianying, Wang Peinan, Li Hui, Zhang Yingjun
Institute of Cereal and Oil Crops, Laboratory of Crop Genetics and Breeding of Hebei, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China.
Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, China.
Front Plant Sci. 2023 Jan 4;13:1098560. doi: 10.3389/fpls.2022.1098560. eCollection 2022.
The primary goal of modern wheat breeding is to develop new high-yielding and widely adaptable varieties. We analyzed four yield-related agronomic traits in 502 wheat accessions under normal conditions (NC) and drought treatment (DT) conditions over three years. The genome-wide association analysis identified 51 yield-related and nine drought-resistance-related QTL, including 13 for the thousand-grain weight (TGW), 30 for grain length (GL), three for grain width (GW), five for spike length (SL) and nine for stress tolerance index (STI) QTL in wheat. These QTL, containing 72 single nucleotide polymorphisms (SNPs), explained 2.23 - 7.35% of the phenotypic variation across multiple environments. Eight stable SNPs on chromosomes 2A, 2D, 3B, 4A, 5B, 5D, and 7D were associated with phenotypic stability under NC and DT conditions. Two of these stable SNPs had association with TGW and STI. Several novel QTL for TGW, GL and SL were identified on different chromosomes. Three linked SNPs were transformed into kompetitive allele-specific PCR (KASP) markers. These results will facilitate the discovery of promising SNPs for yield-related traits and/or drought stress tolerance and will accelerate the development of new wheat varieties with desirable alleles.
现代小麦育种的主要目标是培育新的高产且适应性广泛的品种。我们在三年时间里，对502份小麦种质在正常条件（NC）和干旱处理（DT）条件下的四个与产量相关的农艺性状进行了分析。全基因组关联分析鉴定出51个与产量相关和9个与抗旱性相关的QTL，其中包括小麦中13个与千粒重（TGW）相关的、30个与粒长（GL）相关的、3个与粒宽（GW）相关的、5个与穗长（SL）相关的以及9个与胁迫耐受指数（STI）相关的QTL。这些QTL包含72个单核苷酸多态性（SNP），解释了多个环境中2.23 - 7.35%的表型变异。2A、2D、3B、4A、5B、5D和7D染色体上的8个稳定SNP与NC和DT条件下的表型稳定性相关。其中两个稳定SNP与TGW和STI相关。在不同染色体上鉴定出了几个与TGW、GL和SL相关的新QTL。三个连锁SNP被转化为竞争性等位基因特异性PCR（KASP）标记。这些结果将有助于发现与产量相关性状和/或干旱胁迫耐受性相关的有前景的SNP，并将加速具有理想等位基因的小麦新品种的培育。
