Cristino J A, Pereira A T, Mira M L, Manso C
Instituto Bacteriológico Câmara Pestana, University of Lisbon, Portugal.
J Gen Microbiol. 1987 Jun;133(6):1419-21. doi: 10.1099/00221287-133-6-1419.
A specific method for the identification of Staphylococcus saprophyticus among novobiocin-resistant species isolated from man is described. The test is based on novobiocin resistance, non-fermentation of D-mannose and early inhibition with late secondary growth on glucose/mannose + novobiocin agar plates. On this medium novobiocin-resistant Staphylococcus cohnii showed a confluent, continuous and homogeneous growth after 24 h which remained unchanged at 48 h whether or not it fermented D-mannose, whereas novobiocin-resistant Staphylococcus xylosus fermented D-mannose. These results are discussed in relation to a possible causal role of PTS enzymes and phosphomannose isomerase deficiency in mannose inhibition.
本文描述了一种从人类分离出的耐新生霉素菌株中鉴定腐生葡萄球菌的具体方法。该试验基于对新生霉素的耐药性、不发酵D-甘露糖以及在葡萄糖/甘露糖 + 新生霉素琼脂平板上早期抑制伴后期二次生长。在此培养基上，耐新生霉素的科氏葡萄球菌在24小时后呈现融合、连续且均匀的生长，无论其是否发酵D-甘露糖，在48小时时均保持不变，而耐新生霉素的木糖葡萄球菌发酵D-甘露糖。结合磷酸转移酶（PTS）和磷酸甘露糖异构酶缺乏在甘露糖抑制中可能的因果作用对这些结果进行了讨论。
