Respiratory Diseases Unit, Department of Medical Science, Surgery and Neurosciences, University of Siena, Siena, Italy.
Department of Research and Diagnostics, IRCCS Istituto Giannina Gaslini, Genova, Italy.
Front Immunol. 2023 Jan 4;13:1080556. doi: 10.3389/fimmu.2022.1080556. eCollection 2022.
The characterization of frequency and phenotypes of natural killer (NK) cells and T cells in BAL and peripheral blood of patients with sarcoidosis was evaluated, to discriminate the differential status of these cells in these two compartments. The analysis revealed that CD56CD16 resulted higher in BAL than PB of sarcoidosis and healthy subjects, while CD56CD16 showed a different proportion between BAL and PB of both Sarcoidosis patients and HC. Moreover, in comparison with autologous PB, BAL was characterized by a higher expression of activated NK cell markers NKp44, CD69 and CD25. Significantly increased levels of PD-1 NK cells in the BAL of patients were detected. Regarding the maturation of CD4 and CD8, an increase of Effector Memory T cells (T) was reported in BAL compared to PB. A better characterization of NK and T cells may lead to an improvement of the pathogenetic mechanisms in sarcoidosis.
评估了结节病患者 BAL 和外周血中自然杀伤 (NK) 细胞和 T 细胞的频率和表型特征，以区分这两种细胞在这两个部位的差异状态。分析显示，CD56CD16 在 BAL 中的表达高于结节病和健康受试者的 PB，而 CD56CD16 在结节病患者和 HC 的 BAL 和 PB 之间表现出不同的比例。此外，与自体 PB 相比，BAL 的特征是激活的 NK 细胞标志物 NKp44、CD69 和 CD25 的表达更高。在患者的 BAL 中检测到 PD-1 NK 细胞的水平显著增加。关于 CD4 和 CD8 的成熟，与 PB 相比，BAL 中效应记忆 T 细胞 (T) 的增加。对 NK 和 T 细胞的更好表征可能会改善结节病的发病机制。
