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(鳞翅目:天蛾科)线粒体全基因组序列的特征分析

Characterization of the complete mitochondrial genome sequence of (Lepidoptera: Sphingidae).

作者信息

Meng Yin-Feng, Chen Chao-Fan, Huang Yi-Xin, Wang Xu, Zhang Bo

机构信息

College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, China.

Shanxi Forestry and Grassland Bureau, Taiyuan, China.

出版信息

Mitochondrial DNA B Resour. 2023 Jan 15;8(1):130-132. doi: 10.1080/23802359.2022.2163597. eCollection 2023.

Abstract

In this study, we sequenced and analyzed the complete mitogenome of Ménétriés, 1857. The mitogenome of is a circular structure, and 15,363 bp long in size and encodes 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and a control region (CR). An extremely high AT bias of 79.2% was found in the nucleotide composition of mitogenome. Most of the PCGs used ATN as the start codon and TAA or TAG as the stop codon, which is similar to most other insect mitogenomes, except cox1, which starts with CGA. The phylogeny of Smerinthinae was reconstructed using a maximum-likelihood method, a total of 33 mitogenomes were sampled for phylogenetic analyses. The subfamily Langiinae was selected as outgroup. The results confirmed the position of in the Smerinthinae, in which was placed as the sister taxon to , then to .

摘要

在本研究中,我们对1857年的门氏夜蛾(Ménétriés)的完整线粒体基因组进行了测序和分析。其线粒体基因组为环状结构,大小为15363 bp,编码13个蛋白质编码基因(PCGs)、两个核糖体RNA基因(rRNAs)、22个转运RNA基因(tRNAs)和一个控制区(CR)。在线粒体基因组的核苷酸组成中发现了极高的AT偏向性,为79.2%。大多数PCGs使用ATN作为起始密码子,TAA或TAG作为终止密码子,这与大多数其他昆虫线粒体基因组相似,但cox1除外,其起始密码子为CGA。使用最大似然法重建了天蚕蛾亚科(Smerinthinae)的系统发育,共采集了33个线粒体基因组进行系统发育分析。选择燕蛾亚科(Langiinae)作为外类群。结果证实了该物种在天蚕蛾亚科中的位置,其中该物种被置于与某物种的姐妹分类单元位置,然后是与另一物种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a8a/9848299/e0074e1dcaab/TMDN_A_2163597_F0001_C.jpg

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